Shenmai injection protects mitochondria from oxidative injury in myocardial cells and its mechanism / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 507-513, 2018.
Article
in Zh
| WPRIM
| ID: wpr-775286
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of Shenmai injection on myocardial cells with oxidative injury and the underlying mechanisms.@*METHODS@#Tert-butyl hydroperoxide (t-BHP) was used to induce the oxidative stress in H9c2 myocardial cells. The cell viability and ATP level were evaluated using MTT-colorimetric method and CellTiter-Glo luminescent cell viability assay. The oxygen respiration rate was examined by Clark oxygen electrode. Pyruvate and pyruvate dehydrogenase (PDH) levels were evaluated by ELISA kit. Western blot and quantitative real-time RT-PCR were employed to evaluate the expression of pyruvate dehydrogenase alpha 1(PDHA1) and pyruvate dehydrogenase kinase 1(PDK1).@*RESULTS@#Shenmai injection significantly improved viability and respiration of H9c2 myocardial cells after t-BHP injury (0.05).@*CONCLUSIONS@#Shenmai injection protects mitochondria from oxidative stress by increasing PDH level, which indicates that it may improve energy metabolism of myocardial cells.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Drugs, Chinese Herbal
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Cell Line
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Cell Survival
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Gene Expression Regulation
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Protein Serine-Threonine Kinases
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Oxidative Stress
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Pyruvate Dehydrogenase (Lipoamide)
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Myocytes, Cardiac
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Drug Combinations
Limits:
Animals
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2018
Document type:
Article