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Antineoplastic Effect of Procyanidin-rich Extract of Lafoensia Pacari in Lung Carcinoma Cells
Department of Veterinary MedicineCordeiro, Yonara de Gouveia; Department of Veterinary MedicineRochetti, Arina Lázaro; Department of Biological SciencesSouza, Vinicius Castro; Department of Veterinary MedicineSilva, Edson Roberto da; Department of Basic SciencesScatolini, Antônio Márcio; Department of Food and Experimental NutritionGenovese, Maria Inés; Yasui, George Shigueki; Department of Veterinary MedicineFukumasu, Heidge.
Afiliação
  • Department of Veterinary MedicineCordeiro, Yonara de Gouveia; University of São Paulo. Faculty of Animal Science and Food Engineering. Department of Veterinary MedicineCordeiro, Yonara de Gouveia. Pirassununga. BR
  • Department of Veterinary MedicineRochetti, Arina Lázaro; University of São Paulo. Faculty of Animal Science and Food Engineering. Department of Veterinary MedicineRochetti, Arina Lázaro. Pirassununga. BR
  • Department of Biological SciencesSouza, Vinicius Castro; University of São Paulo. "Escola Superior de Agricultura Luiz de Queiroz". Department of Biological SciencesSouza, Vinicius Castro. Piracicaba. BR
  • Department of Veterinary MedicineSilva, Edson Roberto da; University of São Paulo. Faculty of Animal Science and Food Engineering. Department of Veterinary MedicineSilva, Edson Roberto da. Pirassununga. BR
  • Department of Basic SciencesScatolini, Antônio Márcio; University of São Paulo. Faculty of Animal Science and Food Engineering. Department of Basic SciencesScatolini, Antônio Márcio. Pirassununga. BR
  • Department of Food and Experimental NutritionGenovese, Maria Inés; University of São Paulo. Faculty of Pharmaceutical Science. Department of Food and Experimental NutritionGenovese, Maria Inés. São Paulo. BR
  • Yasui, George Shigueki; National Center for Research and Conservation of Continental Species. Pirassununga. BR
  • Department of Veterinary MedicineFukumasu, Heidge; University of São Paulo. Faculty of Animal Science and Food Engineering. Department of Veterinary MedicineFukumasu, Heidge. Pirassununga. BR
Braz. arch. biol. technol ; 62: e19160638, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1011529
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Lafoensia pacari A. St. Hill has been used in traditional medicine as an anti-ulcerogenic and anti-inflammatory. Although there is an ethnopharmacological indication for cancer treatment, only a few studies have demonstrated its possible anticancer activity. Thus, the aims of this study were (1) to evaluate the antineoplastic effect of L. pacari ethanolic extract (LPE) in lung carcinoma cells, (2) to determine the mode of action of LPE and (3) to identify the substances present in LPE. Human and murine lung cancer cell lines were grown in vitro and treated with different concentrations of LPE. Cell cycle and caspase-3 activity assays were performed in order to verify the mode of action. LC-ESI-MS screening was performed to detect the compounds present in LPE. LPE showed a dose-dependent cytotoxic effect, where neoplastic cells were more sensitive than non-neoplastic. The LPE induced sub-G1 cell cycle arrest in cancer cells suggesting cell death, which was confirmed as apoptosis by the activation of caspase-3. The LC-ESI-MS analysis indicated a high level of procyanidins, which could be responsible for the antineoplastic effect of LPE. Thus, we concluded that a Lafoensia pacari extract, rich in procyanidins, is cytotoxic against lung cancer cells through activation of caspase-3-dependent apoptosis.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Plantas Medicinais / Carcinoma Pulmonar de Células não Pequenas Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: National Center for Research and Conservation of Continental Species/BR / University of São Paulo/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Plantas Medicinais / Carcinoma Pulmonar de Células não Pequenas Idioma: Inglês Revista: Braz. arch. biol. technol Assunto da revista: Biologia Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: National Center for Research and Conservation of Continental Species/BR / University of São Paulo/BR
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