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Use of derived adipose stem cells to reduce complications of cutaneous scarring in smokers. An experimental model in rats
Martins, João Maximiliano Pedron; Oliveira, Fernanda dos Santos de; Lima, Elizabeth Obino Cirne; Dullius, Diego; Durli, Isabel Cirne Lima de Oliveira; Hiraiwa, Everton; Serrano, Tulio; Teixeira, Geciele Rodrigues; Sampaio, Paulo Marcos Ribeiro; Collares, Marcus Vinicius Martins.
Afiliação
  • Martins, João Maximiliano Pedron; Universidade Federal do Rio Grande do Sul. School of Medicine. Postgraduate Program in Surgical Sciences. Porto Alegre. BR
  • Oliveira, Fernanda dos Santos de; Hospital de Clínicas de Porto Alegre. Cell Differentiation Research Center. Laboratory of Embryology. BR
  • Lima, Elizabeth Obino Cirne; HCPA. Plastic Surgery Department. Porto Alegre. BR
  • Dullius, Diego; HCPA. Plastic Surgery Department. Porto Alegre. BR
  • Durli, Isabel Cirne Lima de Oliveira; HCPA. Cell Differentiation Research Center. Laboratory of Embryology. Porto Alegre. BR
  • Hiraiwa, Everton; UFRGS. School of Medicine. Porto Alegre. BR
  • Serrano, Tulio; UFRGS. School of Medicine. Porto Alegre. BR
  • Teixeira, Geciele Rodrigues; HCPA. Laboratory of Embryology and Cell Differentiation Research Center. Porto Alegre. BR
  • Sampaio, Paulo Marcos Ribeiro; HCPA. Cell Differentiation Research Center. Laboratory of Embryology. Porto Alegre. BR
  • Collares, Marcus Vinicius Martins; UFRS. School of Medicine. HCPA. Porto Alegre. BR
Acta cir. bras ; 34(6): e201900605, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1019262
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Purpose To evaluate the use of adipose-derived stem cells (ADSC) in reducing the necrosis area in an experimental model of cutaneous ischemic flap in rats submitted to subcutaneous nicotine injection to simulate a smoker patient. Methods In an experimental study, 30 rats were enrolled and divided into two experimental groups of 15 animals all submitted to a subcutaneous nicotine injection to create ischemic cutaneous flaps on their backs. Other 10 animals were used only to obtain adipose tissue derived stem cells (ADSC). The first group (n=15) received ADSC treatment at the end of surgery while the other group, the control (n=15), received no other interventions. After euthanasia, a decal was performed on the whole area of the flap, accurately defining the transition from necrosis to healthy region. Photos of all animals were collected and evaluated by scales standardized by Paint-Autocad- 2015 software to define the area of flap necrosis in each rat. Student T test was performed to compare the groups, considering a p< 0.05 significant. Data were analyzed using SPSS IBM® 18 version. Results Through the analysis of the images by the program Paint-Autocad-2015 and the area of decal obtained by the transparent sheet, we obtained a mean of 46% necrosis of the total area of the flap in the treatment group and 69.4% in the control group. In the descriptive analysis, a mean of 3.7 cm of necrosis CI 95% (3.2 - 4.2) was evident in the treatment group whereas a mean value of 5.56 CI 95% (5.2 - 5.9) was found in control group, with p value <0.001 for this comparison. Conclusion The application of adipose-derived stem cells reduces the percentage of necrosis in an experimental model of randomized cutaneous flap in rats submitted to subcutaneous nicotine injection.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Tecido Adiposo / Cicatriz / Adipócitos / Necrose / Nicotina Limite: Animais Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: HCPA/BR / Hospital de Clínicas de Porto Alegre/BR / UFRGS/BR / UFRS/BR / Universidade Federal do Rio Grande do Sul/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Tecido Adiposo / Cicatriz / Adipócitos / Necrose / Nicotina Limite: Animais Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: HCPA/BR / Hospital de Clínicas de Porto Alegre/BR / UFRGS/BR / UFRS/BR / Universidade Federal do Rio Grande do Sul/BR
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