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Genetic analysis of Penthorum chinense Pursh by improved RAPD and ISSR in China
Mei, Zhiqiang; Zhang, Xianqin; Khan, Md. Asaduzzaman; Imani, Saber; Liu, Xiaoyan; Zou, Hui; Wei, Chunli; Fu, Junjiang.
Afiliação
  • Mei, Zhiqiang; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Zhang, Xianqin; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Khan, Md. Asaduzzaman; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Imani, Saber; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Liu, Xiaoyan; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Zou, Hui; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Wei, Chunli; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
  • Fu, Junjiang; Southwest Medical University. Research Center for Preclinical Medicine. Key Laboratory of Epigenetics and Oncology. Luzhou. CN
Electron. j. biotechnol ; 30: 6-11, nov. 2017. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1021043
Biblioteca responsável: CL1.1
ABSTRACT

Background:

Penthorum chinense Pursh (P. chinense) is a well-known traditional Chinese medicine (TCM) plant, which has long been used for the prevention and treatment of hepatic diseases. This study aimed to genetically characterize the varieties of P. chinense from different geographic localities of China by random amplification of polymorphic DNA (RAPD)-PCR technique and verified with inter-simple sequence repeat (ISSR) markers.

Results:

The P. chinense samples were collected from nine different geographic localities. Previously improved RAPD and ISSR markers were utilized for genetic analysis using DNA amplification. The genetic relationship dendrogram was obtained by conducting cluster analysis to the similarity coefficient of improved RAPD and ISSR markers. Improved RAPD yielded 185 scorable amplified products, of which 68.6% of the bands were polymorphic, with an average amplification of 9.25 bands per primer. The ISSR markers revealed 156 alleles with 7.8 bands per primers, where 59.7% bands were polymorphic. Furthermore, the similarity coefficient ranges of RAPD and ISSR markers were 0.71­0.91 and 0.66­0.89, respectively.

Conclusions:

This study indicated that improved RAPD and ISSR methods are useful tools for evaluating the genetic diversity and characterizing P. chinense. Our findings can provide the theoretical basis for cultivar identification, standardization, and molecular-assisted breeding of P. chinense for medicinal use.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Plantas Medicinais / Magnoliopsida Tipo de estudo: Estudo diagnóstico / Estudo prognóstico País/Região como assunto: Ásia Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2017 Tipo de documento: Artigo / Documento de projeto País de afiliação: China Instituição/País de afiliação: Southwest Medical University/CN

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Plantas Medicinais / Magnoliopsida Tipo de estudo: Estudo diagnóstico / Estudo prognóstico País/Região como assunto: Ásia Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2017 Tipo de documento: Artigo / Documento de projeto País de afiliação: China Instituição/País de afiliação: Southwest Medical University/CN
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