Development and characterization of InDel markers for Lupinus luteus L. (Fabaceae) and cross-species amplification in other Lupin species
Electron. j. biotechnol
; 31: 44-47, Jan. 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1022247
Biblioteca responsável:
CL1.1
ABSTRACT
Background:
Strong artificial selection and/or natural bottle necks may limit genetic variation in domesticated species. Lupinus luteus, an orphan temperate crop, has suffered diversity reductions during its bitter/sweet alkaloid domestication history, limiting breeding efforts and making molecular marker development a difficult task. The main goal of this research was to generate new polymorphic insertiondeletion (InDel) markers to aid yellow lupin genetics and breeding. By combining genomic reduction libraries and next generation sequencing, several polymorphic InDel markers were developed for L. luteus L.Results:
A total of 118 InDel in silico polymorphic markers were identified. Eighteen InDel primer sets were evaluated in a diverse L. luteus core collection, where amplified between 23 alleles per locus. Observed heterozygosity (HO; 0.0648 to 0.5564) and polymorphic information content (PIC; 0.06 to 0.48) estimations revealed a moderate level of genetic variation across L. luteus accessions. In addition, ten and nine InDel loci amplified successfully Lupinus hispanicus Boiss & Reut, and Lupinus mutabilis Sweet, respectively, two L. luteus close relatives. PCA analysis identified two L. luteus clusters, most likely explained by the domestication species history.Conclusion:
The development of InDel markers will facilitate the study of genetic diversity across L. luteus populations, as well as among closely related species.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Variação Genética
/
Marcadores Genéticos
/
Lupinus
/
Mutação INDEL
/
Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2018
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
/
Estados Unidos
Instituição/País de afiliação:
Brigham Young University/US
/
Centro de Genómica Nutricional Agroacuicola/CL