Optimized protocols for Mycobacterium leprae strain management: frozen stock preservation and maintenance in athymic nude mice
s.l; s.n; 2014. 7 p. ilus, tab.
Não convencional
em Inglês
| Sec. Est. Saúde SP, HANSEN, Hanseníase, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP
| ID: biblio-1095887
Biblioteca responsável:
BR191.1
Localização: BR191.1; 9582/S
ABSTRACT
Leprosy, caused by Mycobacterium leprae, is an important infectious disease that is still endemic in many countries around the world, including Brazil. There are currently no known methods for growing M. leprae in vitro, presenting a major obstacle in the study of this pathogen in the laboratory. Therefore, the maintenance and growth of M. leprae strains are preferably performed in athymic nude mice (NU-Foxn1(nu)). The laboratory conditions for using mice are readily available, easy to perform, and allow standardization and development of protocols for achieving reproducible results. In the present report, we describe a simple protocol for purification of bacilli from nude mouse footpads using trypsin, which yields a suspension with minimum cell debris and with high bacterial viability index, as determined by fluorescent microscopy. A modification to the standard method for bacillary counting by Ziehl-Neelsen staining and light microscopy is also demonstrated. Additionally, we describe a protocol for freezing and thawing bacillary stocks as an alternative protocol for maintenance and storage of M. leprae strains.
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Hanseníase
Base de dados:
HANSEN
/
Hanseníase
/
Sec. Est. Saúde SP
/
SESSP-ILSLACERVO
/
SESSP-ILSLPROD
Assunto principal:
Suspensões
/
Técnicas Bacteriológicas
/
Modelos Animais de Doenças
/
Congelamento
/
Hanseníase
/
Camundongos
/
Camundongos Nus
/
Mycobacterium leprae
Limite:
Animais
Idioma:
Inglês
Ano de publicação:
2014
Tipo de documento:
Não convencional