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Effects of Arf6 downregulation on biological characteristics of human prostate cancer cells
Lei, Haiming; Ma, Fujun; Jia, Renfeng; Tan, Bo.
Afiliação
  • Lei, Haiming; Jiangsu Vocational College of Medicine. School of Clinical Medicine. Yancheng. CN
  • Ma, Fujun; Shengli Oilfield Central Hospital. Department of Urology. Dongying. CN
  • Jia, Renfeng; Shengli Oilfield Central Hospital. Department of Urology. Dongying. CN
  • Tan, Bo; Shengli Oilfield Central Hospital. Department of Urology. Dongying. CN
Int. braz. j. urol ; 46(6): 950-961, Nov.-Dec. 2020. graf
Artigo em Inglês | LILACS | ID: biblio-1134248
Biblioteca responsável: BR1.1
ABSTRACT
ABSTRACT Objective To evaluate the effects of Arf6 downregulation on human prostate cancer cells. Materials and Methods The effects of Arf6 downregulation on cell proliferation, migration, invasion and apoptosis were assessed by MTT, BrdU, scratch, Transwell assays and flow cytometry respectively. AKT, p-AKT, ERK1/2, p-ERK1/2 and Rac1 protein expressions were detected by Western blot. Results Downregulating Arf6 by siRNA interference suppressed the mRNA and protein expressions of Arf6. The proliferation capacities of siRNA group at 48h, 72h, and 96h were significantly lower than those of control group (P <0.05). The migration distance of siRNA group at 18h was significantly shorter than that of control group (P <0.01). The number of cells penetrating Transwell chamber membrane significantly decreased in siRNA group compared with that of control group (P <0.01). After 24h, negative control and normal control groups had similar apoptotic rates (P >0.05) which were both significantly lower than that of siRNA group (P <0.01). After Arf6 expression was downregulated, p-ERK1/2 and Rac1 protein expressions were significantly lower than those of control group (P <0.05). Conclusion Downregulating Arf6 expression can inhibit the proliferation, migration and invasion of prostate cancer cells in vitro, which may be related to ERK1/2 phosphorylation and Rac1 downregulation.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Neoplasia da Próstata Base de dados: LILACS Assunto principal: Neoplasias da Próstata Limite: Humanos / Masculino Idioma: Inglês Revista: Int. braz. j. urol Assunto da revista: Urologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Jiangsu Vocational College of Medicine/CN / Shengli Oilfield Central Hospital/CN

Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Neoplasia da Próstata Base de dados: LILACS Assunto principal: Neoplasias da Próstata Limite: Humanos / Masculino Idioma: Inglês Revista: Int. braz. j. urol Assunto da revista: Urologia Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Jiangsu Vocational College of Medicine/CN / Shengli Oilfield Central Hospital/CN
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