Your browser doesn't support javascript.
loading
Biological effects of formulation containing curcuminoids and Bidens Pilosa L. in oral carcinoma cell line
Arantes, Diego Antonio Costa; Laboratory of Education and Research in Toxicology in VitroSilva, Artur Christian Garcia da; Laboratory of Pharmaceutical TechnologyLima, Eliana Martins; Laboratory of Nanosystems and Modified-Release Drugs DevicesAlonso, Ellen Cristine Pineze; Laboratory of Nanosystems and Modified-Release Drugs DevicesMarreto, Ricardo Neves; Mendonça, Elismauro Francisco; Laboratory of Pharmacology and Cellular ToxicologyValadares, Marize Campos; Batista, Aline Carvalho.
Afiliação
  • Arantes, Diego Antonio Costa; Universidade Federal de Goiás. Dental School. Department of Stomatology (Oral Pathology). Goiânia. BR
  • Laboratory of Education and Research in Toxicology in VitroSilva, Artur Christian Garcia da; Universidade Federal de Goiás. Pharmacy Faculty. Laboratory of Education and Research in Toxicology in VitroSilva, Artur Christian Garcia da. Goiânia. BR
  • Laboratory of Pharmaceutical TechnologyLima, Eliana Martins; Universidade Federal de Goiás. Pharmacy Faculty. Laboratory of Pharmaceutical TechnologyLima, Eliana Martins. Goiânia. BR
  • Laboratory of Nanosystems and Modified-Release Drugs DevicesAlonso, Ellen Cristine Pineze; Universidade Federal de Goiás. Pharmacy Faculty. Laboratory of Nanosystems and Modified-Release Drugs DevicesAlonso, Ellen Cristine Pineze. Goiânia. BR
  • Laboratory of Nanosystems and Modified-Release Drugs DevicesMarreto, Ricardo Neves; Universidade Federal de Goiás. Pharmacy Faculty. Laboratory of Nanosystems and Modified-Release Drugs DevicesMarreto, Ricardo Neves. Goiânia. BR
  • Mendonça, Elismauro Francisco; Universidade Federal de Goiás. Dental School. Department of Stomatology (Oral Pathology). Goiânia. BR
  • Laboratory of Pharmacology and Cellular ToxicologyValadares, Marize Campos; Universidade Federal de Goiás. Pharmacy Faculty. Laboratory of Pharmacology and Cellular ToxicologyValadares, Marize Campos. Goiânia. BR
  • Batista, Aline Carvalho; Universidade Federal de Goiás. Dental School. Department of Stomatology (Oral Pathology). Goiânia. BR
Braz. oral res. (Online) ; 35: e063, 2021. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1249369
Biblioteca responsável: BR1.1
ABSTRACT
Abstract FITOPROT, which contains curcuminoids and Bidens pilosa L. extract, is an innovative mucoadhesive formulation indicated for the topical treatment of chemoradiotherapy-induced oral mucositis (OM) in patients with advanced and visible oral squamous cell carcinoma. The formulation is used as a mouthwash directly on tumor tissue of patients with advanced neoplasms, without triggering cancer cell proliferation or tumor invasiveness. Thus, the aim of this study was to evaluate the biological effects of FITOPROT on an oral squamous cell carcinoma cell line (SCC-4). The viability of SCC-4 cells was assessed after exposure to FITOPROT using MTT reduction assay. The effects of the mucoadhesive formulation on cell cycle progression and cell death parameters were evaluated using flow cytometry. In addition, the inflammatory profile of the tumor cells was evaluated using the cytometric bead array (CBA) assay. FITOPROT promoted a concentration-dependent decrease in cell viability and cell cycle arrest at the G2/M phase (p < 0.05). Mitochondrial membrane potential was also altered after exposure to the formulation (p < 0.05), in parallel with a reduction in VEGF and IL-8 production (p = 0.01 and p = 0.05, respectively). In summary, the results indicate that FITOPROT reduces SCC-4 cell viability, promotes cell cycle arrest, modulates mitochondrial membrane potential, and exhibits antiangiogenic and anti-inflammatory properties, thus indicating its potential for topical use in patients with OM and visible tumors in the mouth.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Neoplasias Bucais e Orofaríngeas Base de dados: BBO - Odontologia / LILACS Assunto principal: Neoplasias Bucais / Carcinoma de Células Escamosas / Bidens Limite: Humanos Idioma: Inglês Revista: Braz. oral res. (Online) Assunto da revista: Odontologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Federal de Goiás/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Neoplasias Bucais e Orofaríngeas Base de dados: BBO - Odontologia / LILACS Assunto principal: Neoplasias Bucais / Carcinoma de Células Escamosas / Bidens Limite: Humanos Idioma: Inglês Revista: Braz. oral res. (Online) Assunto da revista: Odontologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Federal de Goiás/BR
...