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Study of resveratrol against bone loss by using in-silico and in-vitro methods / Estudo do resveratrol contra a perda óssea usando métodos in silico e in vitro
Abbas, S R; Khan, R T; Shafique, S; Mumtaz, S; Khan, A A; Khan, A M; Hassan, Z; Hussain, S A; Abbas, S; Abbas, M R; Batool, A; Safder, M A.
Afiliação
  • Abbas, S R; Karakoram International University. Department of Biological Sciences. Gilgit. PK
  • Khan, R T; University of Azad Jammu and Kashmir. Department of Botany. Azad Jammu and Kashmir. PK
  • Shafique, S; University of Poonch. Department of Plant Breeding and Molecular Genetics. Azad Jammu and Kashmir. PK
  • Mumtaz, S; Karakoram International University. Department of Biological Sciences. Gilgit. PK
  • Khan, A A; Bahauddin Zakariya University. College of Agriculture Bahadur. Layyah. PK
  • Khan, A M; University of Sargodha. Department of Biotechnology. Sargodha. PK
  • Hassan, Z; Bahauddin Zakariya University. College of Agriculture Bahadur. Layyah. PK
  • Hussain, S A; Karakoram International University. Department of Biological Sciences. Gilgit. PK
  • Abbas, S; Karakoram International University. Department of Biological Sciences. Gilgit. PK
  • Abbas, M R; University of Azad Jammu and Kashmir. Department of Computer Sciences and IT. Muzaffarabad. PK
  • Batool, A; Virtual University Lahore. Department of Biotechnology. Lahore. PK
  • Safder, M A; Karakoram International University. Department of Biological Sciences. Gilgit. PK
Braz. j. biol ; 83: e248024, 2023. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1355855
Biblioteca responsável: BR1.1
ABSTRACT
Abstract By applying the in-silico method, resveratrol was docked on those proteins which are responsible for bone loss. The Molecular docking data between the resveratrol and Receptor activator of nuclear factor-kappa-Β ligand [RANKL] receptors proved that resveratrol binds tightly to the receptors, showed the highest binding affinities of −6.9, −7.6, −7.1, −6.9, −6.7, and −7.1 kcal/mol. According to in-vitro data, Resveratrol reduced the osteoclasts after treating Marrow-Derived Macrophages [BMM] with Macrophage colony-stimulating factor [MCSF] 20ng / ml and RANKL 50ng / ml, with different concentrations of resveratrol (2.5, 10 μg / ml) For 7 days, the cells were treated with MCSF (20 ng / ml) and RANKL (40 ng / ml) together with concentrated trimethyl ether and resveratrol (2.5, 10 μg / ml) within 12 hours. Which, not affect cell survival. After fixing osteoclast cells with formaldehyde fixative on glass coverslip followed by incubation with 0.1% Triton X-100 in PBS for 5 min and after that stain with rhodamine phalloidin staining for actin and Hoechst for nuclei. Fluorescence microscopy was performed to see the distribution of filaments actin [F.actin]. Finally, resveratrol reduced the actin ring formation. Resveratrol is the best bioactive compound for drug preparation against bone loss.
RESUMO
Resumo Com a aplicação do método in-silico, o resveratrol foi ancorado nas proteínas responsáveis ​​pela perda óssea. Os dados de docking molecular entre o resveratrol e o ligante do receptor ativador do fator nuclear kappa-Β [Receptor Activator of Nuclear Factor kappa-B Ligant (RANKL)] provaram que o resveratrol se liga fortemente aos receptores, mostraram as afinidades de ligação mais altas de −6,9, −7,6, −7,1, −6,9, - 6,7 e -7,1 kcal / mol. De acordo com dados in-vitro, o resveratrol reduziu os osteoclastos após o tratamento de macrófagos derivados da medula óssea [Bone Marrow-derived Macrophage (BMM)] com fator estimulador de colônias de macrófagos [Macrophage Colony-Stimulating Factor (MCSF)] 20ng / ml e RANKL 50ng / ml, com diferentes concentrações de resveratrol (2,5, 10 μg / ml). Durante sete dias, as células foram tratadas com MCSF (20 ng / ml) e RANKL (40 ng / ml) juntamente com éter trimetílico concentrado e resveratrol (2,5, 10 μg / ml) em 12 horas, processo que não afeta a sobrevivência celular. Após a fixação de células de osteoclastos com fixador de formaldeído em lamela de vidro seguido de incubação com 0,1% Triton X-100 em PBS por 5 min, foi realizado posteriormente o procedimento para corar com rodamina faloidina a actina e Hoechst os núcleos. A microscopia de fluorescência foi realizada para ver a distribuição dos filamentos de actina [F.actina]. Finalmente, o resveratrol reduziu a formação do anel de actina. O resveratrol é o melhor composto bioativo para o preparo de medicamentos contra a perda óssea.
Assuntos


Texto completo: Disponível Base de dados: LILACS / VETINDEX Assunto principal: Osteoclastos / Ligante RANK Idioma: Inglês Revista: Braz. j. biol Ano de publicação: 2023 Tipo de documento: Artigo Instituição/País de afiliação: Bahauddin Zakariya University/PK / Karakoram International University/PK / University of Azad Jammu and Kashmir/PK / University of Poonch/PK / University of Sargodha/PK / Virtual University Lahore/PK

Texto completo: Disponível Base de dados: LILACS / VETINDEX Assunto principal: Osteoclastos / Ligante RANK Idioma: Inglês Revista: Braz. j. biol Ano de publicação: 2023 Tipo de documento: Artigo Instituição/País de afiliação: Bahauddin Zakariya University/PK / Karakoram International University/PK / University of Azad Jammu and Kashmir/PK / University of Poonch/PK / University of Sargodha/PK / Virtual University Lahore/PK
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