A Selective Fluorescent Probe for the Determination and Imaging of Fluoride in Living Cells
Braz. J. Pharm. Sci. (Online)
; 58: e18674, 2022. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1374556
Biblioteca responsável:
BR40.1
Localização: BR40.1
ABSTRACT
Abstract Fluoride anions are indispensable trace elements required for sustaining life. To investigate the homeostasis and action of fluoride in the body, a new highly sensitive and selective fluorescence detection method was designed for fluoride in aqueous solutions. A fluorescent probe for fluoride (FP-F) was synthesized for imaging F- in living cells. The design strategy for the probe was based on the specific reaction between fluoride and silica to mediate deprotection of this probe to fluorescein. Upon treatment with F-, FP-F, a closed and weakly fluorescent lactone, was transformed into an open and strongly fluorescent product. Under the optimum conditions, the detection limit for fluoride was 0.526 nM. FP-F could detect micromolar changes in F- concentrations in living cells by confocal microscopy.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Fluoresceína
/
Fluorescência
/
Flúor
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2022
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Institute of Shandong Xinhua Pharmaceutical Co., Ltd/CN
/
Ludong University/CN
/
Shandong College Of Traditional Chinese Medicine/CN
/
Shandong College of Traditional Chinese Medicine/CN