The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease
Rev. Inst. Med. Trop. Säo Paulo
; 59: e39, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-842782
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Dogs are considered the main reservoir of Leishmania infantum. This protozoan causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil. Serological tests and polymerase chain reaction (PCR) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (Teresina and Vitória). One-hundred infected and 57 non-infected animals from Teresina and 100 non-infected animals from Vitória were studied. Animal selection was not dependent on previous serology. The sensitivity (Teresina) and specificity (Teresina and Vitória) were as follows indirect antibody fluorescence (IFAT) cut-off of 140 (IFAT 140) 96%, 18%, and 76%; IFAT 180 90%, 33%, and 93%; direct agglutination test (DAT) 96%, 33%, and 98%; fast agglutination screening test (FAST) 93%, 68%, and 100%; immunochromatographic assay with a recombinant rK39 antigen (rK39) 88%, 74%, and 98%; enzyme linked immunosorbent assay (ELISA) 91%, 79%, and 98%; rapid dual-path platform test (TR DPP®) 98%, 60%, and 98%; and blood PCR 29%, 93%, and 97%, respectively. In the high transmission area, none of the tests adequately discriminated L. infantum-infected from non-infected dogs. However, in the high transmission city, the area under the receiver operating characteristic (ROC) curve of FAST, DAT, ICrK39, ELISA and TR DPP® was high.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Anticorpos Antiprotozoários
/
Técnicas Imunológicas
/
Leishmania infantum
/
Doenças do Cão
/
Leishmaniose Visceral
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
/
Estudo prognóstico
/
Fatores de risco
/
Estudo de rastreamento
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
Medicina Tropical
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Piauí/BR