Corneal angiogenesis based on different protocols of alkaline cauterization in murine models
Acta cir. bras
; 32(8): 607-616, Aug. 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-886227
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models.Methods:
Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (31). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer's lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed.Results:
Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups.Conclusions:
The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Cauterização
/
Neovascularização da Córnea
/
Compostos de Potássio
/
Modelos Animais de Doenças
/
Neovascularização Patológica
/
Nitratos
Tipo de estudo:
Estudo de avaliação
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Esatdual de São Paulo/BR