The protection effect and mechanism of hyperbaric oxygen therapy in rat brain with traumatic injury
Acta cir. bras
; 33(4): 341-353, Apr. 2018. graf
Artigo
em Inglês
| LILACS
| ID: biblio-886284
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the effect of hyperbaric oxygen therapy (HBOT) on traumatic brain injury (TBI) outcome.Methods:
The modified Marmarou's weight drop device was used to generate non-lethal moderate TBI rat model, and further developed in vitro astrocytes culturing system. Then, we analyzed the expression changes of interested genes and protein by quantitative PCR and western blot.Results:
Multiple HBO treatments significantly reduced the expression of apoptosis promoting genes, such as c-fos, c-jun, Bax and weakened the activation of Caspase-3 in model rats. On the contrary, HBOT alleviated the decrease of anti-apoptosis gene Bcl-2 and promoted the expression of neurotrophic factors (NTFs), such as NGF, BDNF, GDNF and NT-3 in vivo. As a consequent, the neuropathogenesis was remarkably relied with HBOT. Astrocytes from TBI brain or those cultured with 21% O2 density expressed higher NTFs than that of corresponding controls, from sham brain and cultured with 7% O2, respectively. The NTFs expression was the highest in astrocytes form TBI brain and cultured with 21% O2, suggesting a synergistic effect existed between TBI and the following HBO treatment in astrocytes.Conclusion:
Our findings provided evidence for the clinical usage of HBO treating brain damages.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Lesões Encefálicas Traumáticas
/
Oxigenoterapia Hiperbárica
Tipo de estudo:
Estudo de avaliação
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Shanghai Sixth People's Hospital East/CN