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Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells
Du, Qing; Liu, Jia; Zhang, Xuezhi; Zhang, Xin; Zhu, He; Wei, Ming; Wang, Shilei.
Afiliação
  • Du, Qing; Qingdao University. Department of Anesthesiology. Qingdao. CN
  • Liu, Jia; Qingdao University. Department of Anesthesiology. Qingdao. CN
  • Zhang, Xuezhi; Qingdao University. Department of Anesthesiology. Qingdao. CN
  • Zhang, Xin; Qingdao University. Department of Anesthesiology. Qingdao. CN
  • Zhu, He; Qingdao University. Department of Anesthesiology. Qingdao. CN
  • Wei, Ming; Qingdao University. Department of Anesthesiology. Qingdao. CN
  • Wang, Shilei; Qingdao University. Department of Anesthesiology. Qingdao. CN
Braz. j. med. biol. res ; 51(4): e6803, 2018. graf
Article em En | LILACS | ID: biblio-889059
Biblioteca responsável: BR1.1
ABSTRACT
Propofol is an intravenous sedative hypnotic agent of which the growth-inhibitory effect has been reported on various cancers. However, the roles of propofol in endometrial cancer (EC) remain unclear. This study aimed to explore the effects of propofol on EC in vitro and in vivo. Different concentrations of propofol were used to treat Ishikawa cells. Colony number, cell viability, cell cycle, apoptosis, migration, and invasion were analyzed by colony formation, MTT, flow cytometry, and Transwell assays. In addition, the pcDNA3.1-Sox4 and Sox4 siRNA plasmids were transfected into Ishikawa cells to explore the relationship between propofol and Sox4 in EC cell proliferation. Tumor weight in vivo was measured by xenograft tumor model assay. Protein levels of cell cycle-related factors, apoptosis-related factors, matrix metalloproteinases 9 (MMP9), matrix metalloproteinases 2 (MMP2) and Wnt/β-catenin pathway were examined by western blot. Results showed that propofol significantly decreased colony numbers, inhibited cell viability, migration, and invasion but promoted apoptosis in a dose-dependent manner in Ishikawa cells. Moreover, propofol reduced the expression of Sox4 in a dose-dependent manner. Additionally, propofol significantly suppressed the proportions of Ki67+ cells, but Sox4 overexpression reversed the results. Furthermore, in vivo assay results showed that propofol inhibited tumor growth; however, the inhibitory effect was abolished by Sox4 overexpression. Moreover, propofol inhibited Sox4 expression via inactivation of Wnt/β-catenin signal pathway. Our study demonstrated that propofol inhibited cell proliferation, migration, and invasion but promoted apoptosis by regulation of Sox4 in EC cells. These findings might indicate a novel treatment strategy for EC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Propofol / Neoplasias do Endométrio / Apoptose / Fatores de Transcrição SOXC / Hipnóticos e Sedativos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Propofol / Neoplasias do Endométrio / Apoptose / Fatores de Transcrição SOXC / Hipnóticos e Sedativos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China País de publicação: Brasil