Homology modeling and epitope prediction of Der f 33
Braz. j. med. biol. res
; 51(5): e6213, 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-889085
Biblioteca responsável:
BR1.1
ABSTRACT
Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34-45, 63-67, 103-108, 224-230, 308-316, and 365-377) and four T cell epitopes (positions 178-186, 241-249, 335-343, and 402-410) were predicted. These results established a theoretical basis for further studies and eventual epitope-based vaccine design against Der f 33.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Tubulina (Proteína)
/
Alérgenos
/
Epitopos de Linfócito T
/
Epitopos de Linfócito B
/
Dermatophagoides farinae
/
Antígenos de Dermatophagoides
Tipo de estudo:
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2018
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Jiangsu Vocational College of Medicine/CN