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microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb
Yang, Shaofeng; Sheng, Nan; Pan, Lili; Cao, Jing; Liu, Jiao; Ma, Ran.
Afiliação
  • Yang, Shaofeng; Jining No. 1 People's Hospital. Department of Gastroenterology. Jining. CN
  • Sheng, Nan; Jining No. 1 People's Hospital. Department of Gastroenterology. Jining. CN
  • Pan, Lili; Jining No. 1 People's Hospital. Department of Gastroenterology. Jining. CN
  • Cao, Jing; Jining No. 1 People's Hospital. Department of Gastroenterology. Jining. CN
  • Liu, Jiao; Jining No. 1 People's Hospital. Department of Gastroenterology. Jining. CN
  • Ma, Ran; Jining No. 1 People's Hospital. Department of Gastroenterology. Jining. CN
Braz. j. med. biol. res ; 51(6): e6452, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889104
Biblioteca responsável: BR1.1
ABSTRACT
Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A significant up-regulation of miR-3129 was observed in GC tissues compared to adjacent tissues. Overexpression of miR-3129 significantly improved cell viability after 4 days of post-transfection. Flow cytometry assay results showed that the miR-3129 overexpression arrested more SGC7901 cells at S phase. Moreover, overexpression of miR-3129 down-regulated the expression of CDK2 inhibitors while it up-regulated the expression levels of cyclin E, CDK2, and pRb. Interestingly, we found that pRb inhibition reversed the effect of miR-3129 inhibitor on cell proliferation in SGC7901 cells, increased cell viability, reduced cells at G0/1 phase, and modulated the expression of proliferation-related factors. Our results revealed that miR-3129 functioned as an oncogene through positive regulation of pRb and may prove to be a promising option for molecular therapy of GC.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Neoplasias Gástricas / Proteína do Retinoblastoma / Proliferação de Células Limite: Adulto / Feminino / Humanos / Masculino Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Jining No. 1 People's Hospital/CN

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Neoplasias Gástricas / Proteína do Retinoblastoma / Proliferação de Células Limite: Adulto / Feminino / Humanos / Masculino Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Jining No. 1 People's Hospital/CN
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