Removal and killing of multispecies endodontic biofilms by N-acetylcysteine
Braz. j. microbiol
; 49(1): 184-188, Jan.-Mar. 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-889206
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100 mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy. The biofilm viability was measured by viable cell counts and ATP-bioluminescence assay. NAC showed greater efficacy in biofilm cell removal and killing than the other root canal medicaments. Furthermore, 100 mg/mL NAC disrupted the mature multispecies endodontic biofilms completely. These results demonstrate the potential use of NAC in root canal treatment.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Acetilcisteína
/
Streptococcus mutans
/
Actinomyces
/
Enterococcus faecalis
/
Biofilmes
/
Doenças da Polpa Dentária
/
Ligilactobacillus salivarius
/
Antibacterianos
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2018
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Coréia do Sul
Instituição/País de afiliação:
Kyung Hee University/KR