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Prevalence of HPV and EBV infection andtheir relationship with the p53 and PCNA expression in oral carcinoma patients
Veitía, Dayahindara; Liuzzi, Juan; Ávila, Maira; De Guglielmo, Zoraya; Correnti, María.
Afiliação
  • Veitía, Dayahindara; Instituto de Oncología y Hematología-MPPS. Laboratorio de Genética Molecular. Caracas. VE
  • Liuzzi, Juan; Hospital Oncológico ‘Padre Machado’. Servicio de cabeza y cuello. Caracas. VE
  • Ávila, Maira; Hospital Oncológico ‘Padre Machado’. Servicio de cabeza y cuello. Caracas. VE
  • De Guglielmo, Zoraya; Instituto de Oncología y Hematología-MPPS. Laboratorio de Genética Molecular. Caracas. VE
  • Correnti, María; Instituto de Investigaciones Odontológicas Raúl Vincentelli. Facultad de Odontología de la UCV. Caracas. VE
J. oral res. (Impresa) ; 6(4): 86-91, Apr. 2017. tab
Artigo em Inglês | LILACS | ID: biblio-907721
Biblioteca responsável: CL30.1
ABSTRACT

Introduction:

Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions.

Objective:

To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma.

Methodology:

Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the INNO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining.

Results:

The mean age of the patients was 60.55 +/- 13.94 years, and 52 percent of these were female. Of the patients, 59 percent were tobacco users and 63 percent were alcohol consumers. HPV was detected in 70 percent of the patients with the predominance of genotype 16 (60 percent). As for EBV infection, it was observed in 59 percent of cases. p53 and PCNA immunopositivity corresponded to 44 percent and 59 percent, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48 percent of the cases presented infection by both viruses.

Conclusion:

HPV and EBV infection together with the expression of p53 and PCNA were more frequently observed in advanced stages of the disease, suggesting a more relevant role in the progression than in tumor genesis.
Assuntos

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Papillomaviridae / Neoplasias Bucais / Carcinoma de Células Escamosas Tipo de estudo: Estudo de prevalência Limite: Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: J. oral res. (Impresa) Assunto da revista: Odontologia Ano de publicação: 2017 Tipo de documento: Artigo / Documento de projeto País de afiliação: Venezuela Instituição/País de afiliação: Hospital Oncológico ‘Padre Machado’/VE / Instituto de Investigaciones Odontológicas Raúl Vincentelli/VE / Instituto de Oncología y Hematología-MPPS/VE

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Papillomaviridae / Neoplasias Bucais / Carcinoma de Células Escamosas Tipo de estudo: Estudo de prevalência Limite: Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: J. oral res. (Impresa) Assunto da revista: Odontologia Ano de publicação: 2017 Tipo de documento: Artigo / Documento de projeto País de afiliação: Venezuela Instituição/País de afiliação: Hospital Oncológico ‘Padre Machado’/VE / Instituto de Investigaciones Odontológicas Raúl Vincentelli/VE / Instituto de Oncología y Hematología-MPPS/VE
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