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Immunohistochemical Analysis of Cell Proliferation and Bcl-2 Expression in Drug-Induced Gingival Overgrowth
Batista, Ana Luzia Araújo; Mendonça, Angélica Kercya Pereira de; Freitas, Roseana de Almeida; Alves, Pollianna Muniz; Godoy, Gustavo Pina; Nonaka, Cassiano Francisco Weege; Lins, Ruthinéia Diógenes Alves Uchôa.
Afiliação
  • Batista, Ana Luzia Araújo; s.af
  • Mendonça, Angélica Kercya Pereira de; s.af
  • Freitas, Roseana de Almeida; Federal University of Rio Grande do Norte. Natal. BR
  • Alves, Pollianna Muniz; State University of Paraíba. Campina Grande. BR
  • Godoy, Gustavo Pina; Federal University of Pernambuco. Recife. BR
  • Nonaka, Cassiano Francisco Weege; State University of Paraíba. Campina Grande. BR
  • Lins, Ruthinéia Diógenes Alves Uchôa; Federal University of Rio Grande do Norte. Natal. BR
Pesqui. bras. odontopediatria clín. integr ; 17(1): e3331, 13/01/2017. tab, ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-914241
Biblioteca responsável: BR1822.9
ABSTRACT

Objective:

To compare the rate of cell proliferation and expression of antiapoptotic protein Bcl-2 between drug-induced gingival overgrowth (DIGO) and clinical healthy gingiva (CHG) and to establish associations with histopathological features. Material and

Methods:

Twenty specimens of DIGO and 20 CHG specimens were submitted to morphological and immunohistochemical analysis by light microscopy. Cell proliferation (Ki-67) and the expression of Bcl-2 were evaluated in epithelial cells and spindle-shaped mononuclear cells of the connective tissue by establishing the labeling index (LI).

Results:

In epithelial tissue, the mean LI for Ki-67 was 17.2% in DIGO and 21.71% in CHG (p = 0.137). The mean LIs for Bcl-2 in epithelial tissue were 14.67% and 10.24% in DIGO and CHG, respectively (p = 0.026). In connective tissue, DIGO and CHG specimens exhibited low LIs for Ki-67 and Bcl-2, with mean values of less than 0.5% in both groups. No significant differences in the LIs for Ki-67 or Bcl-2 in epithelial tissue were observed according to the degree of collagenization, degree of vascularization and intensity of inflammatory infiltration (p > 0.05). No significant correlations were observed between the LIs for Ki-67 and Bcl-2 (p > 0.05).

Conclusion:

The present results suggest that the pathogenesis of DIGO does not involve increased proliferation or decreased apoptosis of fibroblasts. On the other hand, the morphological pattern of elongated epithelial cristae observed in DIGO could mainly be due to the inhibition of keratinocyte apoptosis and not to increased proliferation of these cells.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: BBO - Odontologia / LILACS Assunto principal: Imuno-Histoquímica / Antígeno Ki-67 / Genes bcl-2 / Proliferação de Células / Fibromatose Gengival País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: Pesqui. bras. odontopediatria clín. integr Assunto da revista: Odontologia Ano de publicação: 2017 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Federal University of Pernambuco/BR / Federal University of Rio Grande do Norte/BR / State University of Paraíba/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: BBO - Odontologia / LILACS Assunto principal: Imuno-Histoquímica / Antígeno Ki-67 / Genes bcl-2 / Proliferação de Células / Fibromatose Gengival País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: Pesqui. bras. odontopediatria clín. integr Assunto da revista: Odontologia Ano de publicação: 2017 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Federal University of Pernambuco/BR / Federal University of Rio Grande do Norte/BR / State University of Paraíba/BR
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