Antifungal Activity, Phytochemical Characterization and Thermal Profile of Anadenanthera colubrina (Vell. ) Brenan
Pesqui. bras. odontopediatria clín. integr
; 17(1): e3389, 13/01/2017. tab, ilus, graf
Artigo
em Inglês
| LILACS, BBO - Odontologia
| ID: biblio-914260
Biblioteca responsável:
BR1822.9
ABSTRACT
Objective:
To investigate the antifungal potential of A. colubrina, and its phytochemical characteristics, thermal profile and toxicity. Material andMethods:
To assess potential antifungal activity, the technique of microdilution was used with the determination of the Minimum Inhibitory Concentration and Minimum Fungicidal Concentration, using standard species of Candida and recent clinical isolates of Candida albicans. Analyses of action of the extract were performed on the wall and cell morphology of C. albicans, of the interactive effect between the plant extract and nystatin on C. albicans through the checkerboard method, and of growth kinetics. The phytochemical screening was determined by spectrophotometry. The thermal profile was traced with the determination of thermogravimetric curves (TG) and differential scanning calorimetry (DSC). The toxicity was evaluated by the method of hemolysis.Results:
The extract of A. colubrina showed a fungistatic potential against all bacteria tested and it acted by modifying the cellular morphology of C. albicans. There was a synergism between nystatin and the plant extract (FIC=0.375), and 53.18% of total polyphenols were determined. The TG curve showed the occurrence of three steps of thermal decomposition. None of the tested concentrations became the effective cytotoxic concentration.Conclusion:
Further studies should be conducted to understand the efficacy and the mechanisms of action involved in the antifungal activity of the plant extract of A. colubrina in order to produce a new drug for the treatment of oral candidiasis.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
BBO - Odontologia
/
LILACS
Assunto principal:
Plantas Medicinais
/
Candida albicans
/
Extratos Vegetais
/
Antifúngicos
Tipo de estudo:
Estudo de avaliação
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2017
Tipo de documento:
Artigo