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Differential role of gpaB and sidA gene expressions in relation to virulence in Aspergillus species from patients with invasive aspergillosis
Ghods, Nayereh; Falahati, Mehraban; Roudbary, Maryam; Farahyar, Shirin; Shamaei, Masoud; Pourabdollah, Mahin; Seif, Farhad.
Afiliação
  • Ghods, Nayereh; Iran University of Medical Sciences. School of Medicine. Department of Medical Mycology and Parasitology. Tehran. IR
  • Falahati, Mehraban; Iran University of Medical Sciences. School of Medicine. Department of Medical Mycology and Parasitology. Tehran. IR
  • Roudbary, Maryam; Iran University of Medical Sciences. School of Medicine. Department of Medical Mycology and Parasitology. Tehran. IR
  • Farahyar, Shirin; Iran University of Medical Sciences. School of Medicine. Department of Medical Mycology and Parasitology. Tehran. IR
  • Shamaei, Masoud; Shahid Beheshti University of Medical Sciences. National Research Institute of Tuberculosis and Lung Diseases. Clinical Tuberculosis and Epidemiology Research Center. Tehran. IR
  • Pourabdollah, Mahin; Shahid Beheshti University of Medical Sciences. Chronic Respiratory Diseases Research Center. National Research Institute of Tuberculosis and Lung Diseases. Tehran. IR
  • Seif, Farhad; Iran University of Medical Sciences. School of Medicine. Department of Immunology. Tehran. IR
Braz. j. microbiol ; 49(3): 668-674, July-Sept. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-951814
Biblioteca responsável: BR1.1
ABSTRACT
Abstract The virulence genes in invasive aspergillosis (IA) have not been analyzed adequately. The present study was designed to evaluate the expression of gpaB and sidA genes, which are important virulence genes in Aspergillus spp. from bronchoalveolar lavage (BAL) samples. Direct examination and culture on Czapek Agar and Sabouraud Dextrose Agar media were performed for 600 BAL specimens isolated from patients with possible aspergillosis. A Galactomannan ELISA assay was also carried out. The expression levels of the gpaB and sidA genes in isolates were analyzed using quantitative real-time PCR (qRT-PCR). We identified 2 species, including Aspergillus flavus (A. flavus) and Aspergillus fumigatus (A. fumigatus) in 25 positive samples for invasive aspergillosis as validated using GM-ELISA. A. flavus is the main pathogen threatening transplant recipients and cancer patients worldwide. In this study, A. flavus had low levels of the gpaB gene expression compared to A. fumigatus (p = 0.006). The highest sidA expression was detected in transplant recipients (p = 0.05). There was no significant correlation between sidA expression and underlying disease (p = 0.15). The sidA and gpaB gene expression patterns may provide evidence that these virulence genes play important roles in the pathogenicity of Aspergillus isolates; however, there are several regulatory genes responsible for the unexpressed sidA and gpaB genes in the isolates.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Aspergilose / Aspergillus flavus / Aspergillus fumigatus / Proteínas de Bactérias Limite: Adulto / Idoso / Idoso, 80 anos ou mais / Feminino / Humanos / Masculino Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Irã Instituição/País de afiliação: Iran University of Medical Sciences/IR / Shahid Beheshti University of Medical Sciences/IR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Aspergilose / Aspergillus flavus / Aspergillus fumigatus / Proteínas de Bactérias Limite: Adulto / Idoso / Idoso, 80 anos ou mais / Feminino / Humanos / Masculino Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Irã Instituição/País de afiliação: Iran University of Medical Sciences/IR / Shahid Beheshti University of Medical Sciences/IR
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