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Evaluation of the therapeutic effect of methylene blue on the liver of rats submitted to ischemia and reperfusion
Castro-e-Silva, Orlando; D'Albuquerque, Luiz Augusto Carneiro; Silveira, Marina Rodrigues Garcia; Zorzi, Patricia; Liu, Jordan Bistafa; Campos, Daniel Tófoli Queiroz; Victorino, João Paulo; Jordani, Maria Cecília; Mendes, Karina Dal Sasso; Évora, Paulo Roberto Barbosa.
Afiliação
  • Castro-e-Silva, Orlando; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Surgery and Anatomy Department. Ribeirao Preto. BR
  • D'Albuquerque, Luiz Augusto Carneiro; USP. Medical School. Department of Gastroenterology. Sao Paulo. BR
  • Silveira, Marina Rodrigues Garcia; USP. Sao Paulo Medical School. Department of Preventive Medicine. Sao Paulo. BR
  • Zorzi, Patricia; USP. FMRP. Ribeirao Preto. BR
  • Liu, Jordan Bistafa; Faculdade de Medicina de Marília. Marilia. BR
  • Campos, Daniel Tófoli Queiroz; Faculdade de Medicina de Marília. Marilia. BR
  • Victorino, João Paulo; USP. Ribeirão Preto College of Nursing. Ribeirao Preto. BR
  • Jordani, Maria Cecília; USP. FMRP. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Mendes, Karina Dal Sasso; USP. General and Specialized Nursing Department. Ribeirao Preto. BR
  • Évora, Paulo Roberto Barbosa; USP. FMRP. Department of Surgery and Anatomy. Ribeirao Preto. BR
Acta cir. bras ; 33(12): 1043-1051, Dec. 2018. graf
Artigo em Inglês | LILACS | ID: biblio-973484
Biblioteca responsável: BR1.1
ABSTRACT
Abstract

Purpose:

To analyze the effect of methylene blue (MB) therapy during the liver ischemia-reperfusion injury (I/R) process.

Methods:

Thirty-five male Wistar rats were used, (70%) submitted to partial ischemia (IR) or not (NIR) (30%) were obtained from the same animal. These animals were divided into six groups 1) Sham (SH), 2) Sham with MB (SH-MB); 3) I/R, submitted to 60 minutes of partial ischemia and 15 minutes of reperfusion; 4) NI/R, without I/R obtained from the same animal of group I/R; 5) I/R-MB submitted to I/R and MB and 6) NI/R-MB, without I/R. Mitochondrial function was evaluated. Osmotic swelling of mitochondria as well as the determination of malondialdehyde (MDA) was evaluated. Serum (ALT/AST) dosages were also performed. MB was used at the concentration of 15mg/kg, 15 minutes before hepatic reperfusion. Statistical analysis was done by the Mann Whitney test at 5%.

Results:

State 3 shows inhibition in all ischemic groups. State 4 was increased in all groups, except the I/R-MB and NI/R-MB groups. RCR showed a decrease in all I/R and NI/R groups. Mitochondrial osmotic swelling showed an increase in all I/R NI/R groups in the presence or absence of MB. About MDA, there was a decrease in SH values in the presence of MB and this decrease was maintained in the I/R group. AST levels were increased in all ischemic with or without MB.

Conclusions:

The methylene blue was not able to restore the mitochondrial parameters studied. Also, it was able to decrease lipid peroxidation, preventing the formation of reactive oxygen species.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Traumatismo por Reperfusão / Inibidores Enzimáticos / Fígado / Azul de Metileno Tipo de estudo: Estudo de avaliação Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Faculdade de Medicina de Marília/BR / USP/BR / Universidade de São Paulo/BR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Traumatismo por Reperfusão / Inibidores Enzimáticos / Fígado / Azul de Metileno Tipo de estudo: Estudo de avaliação Limite: Animais / Humanos / Masculino Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Faculdade de Medicina de Marília/BR / USP/BR / Universidade de São Paulo/BR
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