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Tissue microarray technology and collagen evaluation to analyze surgical trauma performed with usual blade or ultrasonic harmonic scalpels in rats
Costa-Filho, Octavio Antonio Azevedo da; Costa, Mario Augusto Cray da; Waaga-Gasser, Ana Maria; Kubrusly, Luiz Fernando; Oliveira, Luciane Bugmann Moreira de; Collaço, Luiz Martins; Magalhães, Maria Angélica Baron; Gasser, Martin; Malafaia, Osvaldo; Ribas-Filho, Jurandir Marcondes.
Afiliação
  • Costa-Filho, Octavio Antonio Azevedo da; Faculdade Evangélica do Paraná. Postgraduate Program in Principles of Surgery. Curitiba. BR
  • Costa, Mario Augusto Cray da; Universidade Estadual de Ponta Grossa. Department of Medicine. BR
  • Waaga-Gasser, Ana Maria; Harvard Medical School. Brigham and Women's Hospital. Transplantation Research Center. Boston. US
  • Kubrusly, Luiz Fernando; FEPAR. Postgraduate Program in Principles of Surgery. Curitiba. BR
  • Oliveira, Luciane Bugmann Moreira de; FEPAR. Postgraduate Program in Principles of Surgery. Curitiba. BR
  • Collaço, Luiz Martins; FEPAR. Postgraduate Program in Principles of Surgery. Curitiba. BR
  • Magalhães, Maria Angélica Baron; FEPAR. Postgraduate Program in Principles of Surgery. Curitiba. BR
  • Gasser, Martin; University of Wuerzburg. Department of Surgery I. Wuerzburg. DE
  • Malafaia, Osvaldo; FEPAR. Postgraduate Program in Principles of Surgery. Curitiba. BR
  • Ribas-Filho, Jurandir Marcondes; FEPAR. Postgraduate Program in Principles of Surgery. Curitiba. BR
Acta cir. bras ; 33(9): 762-774, Sept. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-973507
Biblioteca responsável: BR1.1
ABSTRACT
Abstract

Purpose:

To compare wound healing performed with cold blade (CSB) and ultrasonic harmonic scalpel (UHS) in the abdominal aponeurosis of rats.

Methods:

Eighty Wistar rats divided into two groups and underwent midline incision in the linea alba with cold blade and harmonic ultrasonic scalpel. Analysis were performed in subgroups of 10 animals after 3, 7, 14 and 21 days. Macroscopically was observed the presence of hematoma, infection, wound dehiscence, fistula and adherences. Microscopically were used collagen and immunohistochemical staining methods.

Results:

Macroscopic, complications showed no statistical difference. Immunohistochemical analysis for MMP-9 was more intense in UHS group (p<0.05). TGF β presented its lower expression in UHS group at 14 and 21 days, with no statistical difference at 3 and 7 days (p<0.05). α-AML expression appeared higher in UHS group after 14 days and remained similar in others (p<0.05). Collagen deposition had no change in type I, and increased in type III in UHS; at 7th day the deposition was higher in CSB group; at 14th was similar in both groups (p<0.001).

Conclusion:

UHS compared to the CSB has higher lesion area at the time of the incision; as well as it led to the delay of regeneration and scar maturation process.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Cicatrização / Colágeno / Parede Abdominal / Ferida Cirúrgica Limite: Animais Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Brasil / Alemanha / Estados Unidos Instituição/País de afiliação: FEPAR/BR / Faculdade Evangélica do Paraná/BR / Harvard Medical School/US / Universidade Estadual de Ponta Grossa/BR / University of Wuerzburg/DE

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Cicatrização / Colágeno / Parede Abdominal / Ferida Cirúrgica Limite: Animais Idioma: Inglês Revista: Acta cir. bras Assunto da revista: CIRURGIA GERAL / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Brasil / Alemanha / Estados Unidos Instituição/País de afiliação: FEPAR/BR / Faculdade Evangélica do Paraná/BR / Harvard Medical School/US / Universidade Estadual de Ponta Grossa/BR / University of Wuerzburg/DE
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