Vitamin C inhibits crystallization of struvite from artificial urine in the presence of Pseudomonas aeruginosa
Int. braz. j. urol
; 44(6): 1234-1242, Nov.-Dec. 2018. graf
Artigo
em Inglês
| LILACS
| ID: biblio-975655
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Background:
Formation of struvite stones is associated with urinary tract infection by urease-producing bacteria. Biogenic crystal growth in natural and synthetic materials is regulated by the action of inhibitors, ranging from small ions, molecules to large macromolecules. Materials andMethods:
We report the dynamics of in vitro crystallization of struvite in presence of vitamin C in synthetic urine using single diffusion gel growth technique. Sodium metasilicate gel of specific gravity 1.05 and the aqueous solution of ammonium dihydrogen phosphate were used as the medium for growing the struvite crystals. The crystallization process was induced by a urease positive struvite stone associated Pseudomonas aeruginosa to mimic the infection leading to stone formation. The grown crystals were characterized by ATR-FTIR and powder XRD. The surface morphology was analysed through FE-SEM for comparison between treatments.Results:
We observed decrease in number, dimension, and growth rate of struvite crystals with the increasing concentrations of vitamin C. Crystals displayed well-defined faces and dendritic morphology of struvite in both control and biogenic systems.Conclusion:
The results strongly suggest that, vitamin C can modulate the formation of struvite crystals in the presence of uropathogenic bacteria.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Pseudomonas aeruginosa
/
Ácido Ascórbico
/
Urina
/
Vitaminas
/
Cálculos
/
Estruvita
Limite:
Humanos
Idioma:
Inglês
Revista:
Int. braz. j. urol
Assunto da revista:
Urologia
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Yenepoya University/IN