Undernutrition impairs the quality of growth plate and trabecular and cortical bones in growing rats
Acta cir. bras
; 34(3): e201900301, 2019. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-989070
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the effects of dietary restriction on the growth plate and long bone tissue in growing rats.Methods:
Sixty male Wistar rats were randomly assigned to two groups Control (Con) and Diet-restricted (Res). After weaning, the Res rats were offered 50% of the chow ingested by the control (ad libitum food intake). The animals were subdivided into two subgroups with follow-ups up to 56 or 70 days. After euthanasia, the growth plate of tibias was analyzed by histomorphometry, micro-computed tomography, and mechanical test. The trabecular and compact bones were evaluated by histomorphometry, dual-energy X-ray absorptiometry, and micro-computed tomography (μCT). Real-time PCR was used to analyze gene expression.Results:
Although dietary restriction did not alter gene expression, several phenotypic changes were seen in the growth plate; i.e., decrease in volume, reduction in total area and height, decrease in the area ossified zones, mechanical weakening, reduction in mass of trabecular and cortical bone, lower bone density, deterioration of the trabecular and cortical microarchitecture, and trabeculae with lower collagen deposition.Conclusion:
Dietary restriction had severe detrimental effects on the growth plate and trabecular and cortical bone.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Densidade Óssea
/
Desnutrição
/
Osso Esponjoso
/
Osso Cortical
/
Lâmina de Crescimento
Aspecto:
Determinantes sociais da saúde
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
USP/BR
/
Universidade de Ribeirão Preto/BR
/
Universidade de São Paulo/BR