Your browser doesn't support javascript.
loading
Structural changes of the colonic mucosa induced by orlistat experimental study
Egyptian Journal of Histology [The]. 2010; 33 (4): 635-648
em Inglês | IMEMR | ID: emr-110726
Biblioteca responsável: EMRO
ABSTRACT
Orlistat, an anti-obesity drug, is a lipase inhibitor which increases fecal fat excretion. Many workers had reported the harmful consequences of increased fecal fat excretion on colonic mucosa. So the present study was designed to evaluate the effect of Orlistat in presence of other risk factors [directly related to colon carcinogenesis] as high fat diet and colon carcinogen di-methyl hydrazine on the structure of rat colonic mucosa and cell proliferation evaluated by the PCNA index. The study included 50 male albino rats, which were divided into 5 equal groups. Group 1 served as a control group. Group II received high fat diet alone. Group III received high fat diet and Orlistat [32 mg/ kg] orally for 5 weeks. Group IV were subcutaneously injected by two doses of the carcinogen di-methyl hydrazine [DMH] [25mg/kg] together with high fat diet. While rats of group V received Orlistat, di-methyl hydrazine [DMH] and high fat diet. Histological examination of the colonic mucosa revealed presence of 3 types of structurally-altered crypts. The first type appeared with dilated lumen [typical aberrant crypt]. This type was significantly recognized in group II and III. However, insignificant difference in incidence of aberrant crypts and cell proliferation evaluated by PCNA index was encountered between group II and III. The second type of crypt alteration [hyperplastic aberrant crypt], appeared having a serrated luminal configuration, distended goblet cells and proliferating epithelial foci that might partially or totally occluded the lumen. The third type [dysplastic aberrant crypt] exhibited few goblet cells and/or crowded nuclei with variability in their shape and increase in their length together with frequent mitotic figures. Significant increase in number of hyperplastic and dysplastic aberrant crypts as well as PCNA index was detected in group V compared to group IV, determining the potentiating effect of Orlistat. Long-term use of Orlistat in presence of risk factors, as high fat diet and other predisposing factor for cancer colon, was associated with severe crypt alterations and enhancement of colonic proliferative capacity, putative biomarkers of colon cancer
Assuntos
Buscar no Google
Base de dados: IMEMR Assunto principal: Ratos / Imuno-Histoquímica / Gorduras na Dieta / Fatores de Risco / Colo / Antígeno Nuclear de Célula em Proliferação Limite: Animais Idioma: Inglês Revista: Egypt. J. Histol. Ano de publicação: 2010
Buscar no Google
Base de dados: IMEMR Assunto principal: Ratos / Imuno-Histoquímica / Gorduras na Dieta / Fatores de Risco / Colo / Antígeno Nuclear de Célula em Proliferação Limite: Animais Idioma: Inglês Revista: Egypt. J. Histol. Ano de publicação: 2010
...