Anxiolytic Effect of Herbal medicine, Khamira Gaozaban Ambri Jadwar Ood Salib Wala [KGJ] in experimental rat models

ABSTRACT

Anxiety and depression leads to a number of morbid states. Search of new agents which are low-priced and safe alternative is necessary. Khamira Gaozaban Ambri Jadwar Ood Salib wala [KGJ], is a product of Hamdard Laboratories [Waqf] Pakistan. They claim that it is anxiolytic, anti-convulsant and nervine tonic. However this claim is not scientifically proven. Some components are proved to be anxiolytic but combination may alter the individual properties of drugs. Therefore we designed present study to prove these properties of KGJ scientifically. Thirty male Sprague Dawley rats were divided into five groups of six animals in each group. The groups were saline group, control group [receiving Diazepam I mg/kg] and three test groups receiving 86, 170 and 360 mg/kg doses of Khamira orally. Assessments of different doses of KGJ in comparison to diazepam were carried out on anxiety paradigms namely "Elevated Plus Maze", "Light And Dark Activity Box" and "Open Field" paradigms. KGJ produced anxiolytic effects in all the behavioral methods, which were not significantly different from the effects produced by diazepam. Basal levels of corticosterone not altered by diazepam were decreased by 86 mg/kg dose of KGJ. Same dose of KGJ also decreased blood glucose levels. In conclusion, these results suggest anxiolytic potential of KGJ with non-sedative property