Effect of obesity on lipolytic activity of adipose tissue in male rats

ABSTRACT

Obesity has become a major problem in the modern world; it is affected genetically and by lifestyle. Since obesity is associated with multi co-morbidities like dyslipidemia, hypertension, and metabolic syndrome, this work studied the effect of induced obesity on body weight, blood pressure, lipid profile, glycemic state and lipolytic activity of adipose tissue in male rats. Twenty male rats of a body weight ranging from 178 to 200 g were divided equally into two groups, one fed commercial rat chow as a control group [Cgp] and the other fed 10% saturated fat to induce obesity as experimental group [SFgp]. After 3 months the body weight, blood pressure, fasting blood glucose, insulin level, and lipid profile of both groups were measured and the lipolytic activity of adipose tissue was assessed by the amount of free glycerol released. Rats fed saturated fat for 3 months showed significant increase in birth weight, systolic blood pressure, TG, Cholesterol, LDL, fasting blood glucose and insulin levels by 55.3%, 15.86% 24%, 7.92%, 19.58% 20.5% and 26.25% respectively with a significant decline in HDL by 12.9%. Lipolytic activity of SC tissue in the presence of adrenaline decreased significantly by 15%, while in the presence of insulin it increased significantly by 33.33%. It showed a significant increase by 18% and 25.84% in the presence of adrenaline and insulin respectively in visceral adipose tissue. A p value less than 0.05 was considered statistically significant. obesity induced in male rats by high saturated fat diet showed a significant rise in BW and is associated with hypertension, dyslipidemia, hyperglycemia and insulin resistance. The adipose tissue of obese male rats showed a significant decrease in lipolytic activity of SC with a significant rise in the corresponding value in visceral adipose tissue