Comparative polyphenolic content and antioxidant activities of Genista tinctoria L. and Genistella sagittalis [L.] Gams [fabaceae]

ABSTRACT

The aim of this study was focused on the polyphenolic composition and antioxidant capacity of Genista tinctoria L. and Genistella sagittalis [L.] Gams. A qualitative and quantitative characterization of the main phenolic compounds from the extracts were carried out using a HPLC-MS method. The total polyphenolic and flavonoid content was spectrophotometrically determined. The antioxidant activity towards various radicals generated in different systems was evaluated using DPPH bleaching method, Trolox equivalent antioxidant capacity assay [TEAC] and Oxygen radical absorbance capacity [ORAC], and all indicated that G. tinctoria extract was more antioxidant than G. sagittalis extract. That was in good agreement with the total polyphenolic and flavonoidic content. Chlorogenic acid, p-coumaric acid, isoquercitrin and apigenin were identified in bothspecies. Caffeic acid, ferulic acid, hyperoside, rutin, quercitrin and luteolin were found only in G. tinctoria, while quercetin was determined in G. sagittalis