Culture conditions for zinc- and pH-regulated gene expression studies in Aspergillus fumigatus
Int. microbiol
; 10(3): 187-192, sept. 2007. ilus
Artigo
em En
| IBECS
| ID: ibc-056710
Biblioteca responsável:
ES1.1
Localização: ES1.1 - BNCS
ABSTRACT
In Aspergillus fumigatus, the regulation of zinc homeostasis is strongly influenced by environmental pH. Thus, the study of zinc-regulated gene expression in A. fumigatus requires controlling variations in culture pH, as this may affect zinc availability. However, depending on the nitrogen source, the pH of the culture can change dramatically over time. In addition, due to the ubiquitous distribution of zinc and that it is an essential micronutrient required in minute amounts for optimal fungal growth, neither buffering of the culture media to prevent pH variations nor the use of chelating agents is advisable if mycelium is to be used for expression analyses. In this work, the growth of A. fumigatus in several culture media was examined in order to determine the conditions yielding mycelia suitable for gene expression analyses in acid and neutral media, regardless of zinc availability. Our results showed that a zinc-limiting synthetic basal medium could be readily converted into a zinc-replete one and subsequently into acid or neutral medium by using, respectively, ammonium or nitrate as nitrogen source (AU)
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Aspergillus fumigatus
/
Zinco
/
Meios de Cultura
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2007
Tipo de documento:
Artigo
Instituição/País de afiliação:
University of Salamanca-CSIC/Spain