Variability of the fimA gene in Porphyromonas gingivalis isolated from periodontitis and non-periodontitis patients
Med. oral patol. oral cir. bucal (Internet)
; 18(1): 100-105, ene. 2013. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-108229
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Objective:
The goal of this study was to determine the genetic variability of the fimA gene in Porphyromonas gingivalis isolates from Spanish patients. StudyDesign:
Pooled subgingival samples were taken, processed and cultured in non-selective blood agar medium. Pure cultures of one to six isolates per patient were obtained and PCR and PCR-RFLP were used for fimbrillin gene (fimA) type determination of the extracted genomic (DNA).Results:
Two hundred and twenty four Porphyromonas gingivalis isolates from 65 patients were analyzed consisting of 15 non-periodontitis patients (66 isolates) and 50 with periodontitis (158 isolates). Genotype II was the most prevalent (50.9%), while the other types of fimbriae did not exceed fifteen percent of prevalence. Isolates with types II and IV of fimbriae were significantly more prevalent in periodontitis patients than isolates with genotype I. Co-infection was observed in 17.65% of the patients analyzed.Conclusion:
The results suggest that in this population Porphyromonas gingivalis with type II of fimbriae are significantly more predominant in periodontitis patients than genotype I (AU)RESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Periodontite
/
Porphyromonas gingivalis
Tipo de estudo:
Fatores de risco
Limite:
Humanos
Idioma:
Inglês
Revista:
Med. oral patol. oral cir. bucal (Internet)
Ano de publicação:
2013
Tipo de documento:
Artigo
Instituição/País de afiliação:
Complutense University/Spain
/
DENTAID S.L/Spain