Erythropoietin ameliorates the reduced migration of human fibroblasts during in vitro hypoxia
J. physiol. biochem
; 67(1): 1-13, mar. 2011.
Artigo
em Inglês
| IBECS
| ID: ibc-122629
Biblioteca responsável:
ES1.1
Localização: BNCS
RESUMEN
No disponible
ABSTRACT
Erythropoietin promotes the formation of granulation tissue when administered to soft tissue wounds and it was shown to be most effective under tissue hypoxia. However, the action of erythropoietin on the cellular level is not well understood. In order to get a better insight into these processes, an in vitro wound healing assay was applied. Two main players of soft tissue healingfibroblasts and microvascular endothelial cellswere used as mono- and co-cultures, subsequently inflicting in vitro wounds. Cell migration, proliferation, the differentiation of fibroblasts to myofibroblasts, and the release of vascular endothelial cell growth factor A and angiogenin were quantified in response to hypoxia and erythropoietin (5 IU/ml). Erythropoietin supplementation did neither affect proliferation nor migration of endothelial cells and fibroblasts under normoxia. Under hypoxia, the reduced fibroblast migration was ameliorated by erythropoietin. This effect coincided with an attenuated release of vascular endothelial growth factor A, whereas angiogenin release was unaffected by erythropoietin. The in vitro model applied in this study may represent an adequate approximation to certain aspects of the in vivo status of soft tissue regeneration and the results might serve to interpret the in vivo efficiency of erythropoietin at the cellular level Erythropoietin has different impacts on the cells in normoxia and hypoxia. Its positive influence on fibroblast migration during hypoxia seems to support the strategies of applying erythropoietin in those chronic wounds, which exhibit fibroblastic dysfunction although good vascularisation is present (AU)
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Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Hipóxia Celular
/
Eritropoetina
/
Fibroblastos
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2011
Tipo de documento:
Artigo
Instituição/País de afiliação:
Saarland University/Germany