Oxidative stress in mouse liver caused by dietary amino acid deprivation: protective effect of methionine
J. physiol. biochem
; 66(2): 93-103, jun. 2010.
Artigo
em Inglês
| IBECS
| ID: ibc-122833
Biblioteca responsável:
ES1.1
Localização: BNCS
RESUMEN
No disponible
ABSTRACT
The aim of this work was to evaluate the effects of a diet depleted of amino acids (protein-free diet, or PFD), as well as the supplementation with methionine (PFD+Met), on the antioxidant status of the female mouse liver. With this purpose, cytosolic protein spots from two-dimensional non-equilibrium pH gel electrophoresis were identified by several procedures, such as mass spectrometry, Western blot, gel matching and enzymatic activity. PFD decreased the contents of catalase (CAT),peroxiredoxin I (Prx-I), and glutathione peroxidase (GPx) by 67%, 37% and 45%, respectively. Gene expression analyses showed that PFD caused a decrease in CAT (−20%) and GPx (−30%) mRNAlevels but did not change that of Prx-I. It was also found that, when compared to a normal diet, PFD increased the liver contents of both reactive oxygen species (+50%) and oxidized protein (+88%) and decreased that of glutathione (−45%). Supplementation of PFD with Met prevented these latter effects to varying degrees, whereas CAT, Prx-I and GPx mRNA levels resulted unmodified. Present results suggest that dietary amino acid deprivation deranges the liver antioxidant defences, and this can be, in part, overcome by supplementation with Met (AU)
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Estresse Oxidativo
/
Aminoácidos
/
Fígado
/
Metionina
Limite:
Animais
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2010
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidad Nacional de Mar del Plata-CONICET/Argentina