Identification of valid reference housekeeping genes for gene expression analysis in tumor neovascularization studies
Clin. transl. oncol. (Print)
; 15(3): 211-218, mar. 2013. tab, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-127080
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
INTRODUCTION:
Real time RT-PCR is a widely used technique to evaluate and confirm gene expression data obtained in different cell systems and experimental conditions. However, there are many conflicting reports about the same gene or sets of gene expression. A common method is to report the interest gene expression relative to an internal control, usually a housekeeping gene (HKG), which should be constant in cells independently of experimental conditions. MATERIALS ANDMETHODS:
In this study, the expression stability of ten HKGs was considered in parallel in two cell systems (endothelial and osteosarcoma cells) beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box binding protein (TBP), hypoxanthine phosphoribosyl-transferase 1 (HPRT1), Cyclophilin A (PPIA), beta-2-microglobulin (B2M), glucuronidase beta (GUSB), eukaryotic translation elongation factor 1 alpha1 (EEF1A1), transferrin receptor (TFRC), ribosomal protein S18 (RPS18). In order to study the stability of candidate reference genes, data have been also analyzed by several algorithms (geNorm, NormFinder, BestKeeper and delta-Ct method). RESULTS ANDCONCLUSIONS:
The overall analysis obtained by the comprehensive ranking showed that RPS18 and PPIA are appropriate internal reference genes for tumor neovascularization studies where it is necessary to analyze both systems at the same time (AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Osteoblastos
/
Neoplasias Ósseas
/
Endotélio Vascular
/
Osteossarcoma
/
Genes Essenciais
/
Neovascularização Patológica
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2013
Tipo de documento:
Artigo
Instituição/País de afiliação:
IRCCS SDN Foundation/Italy
/
Second University of Naples/Italy