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Caspase-2 immunohistochemistry in differentiating cells during mouse cephalic development
Vanmuylder, Nathalie; Lejong, Marie; Choa-Duterre, Myriam; Louryan, Stéphane.
Afiliação
  • Vanmuylder, Nathalie; Université Libre de Bruxelles. Faculté de Médecine. Laboratoire d'Anatomie, Biomécanique et Organogenèse. Bruxelles. Belgium
  • Lejong, Marie; Université Libre de Bruxelles. Faculté de Médecine. Laboratoire d'Anatomie, Biomécanique et Organogenèse. Bruxelles. Belgium
  • Choa-Duterre, Myriam; Université Libre de Bruxelles. Faculté de Médecine. Laboratoire d'Anatomie, Biomécanique et Organogenèse. Bruxelles. Belgium
  • Louryan, Stéphane; Université Libre de Bruxelles. Faculté de Médecine. Laboratoire d'Anatomie, Biomécanique et Organogenèse. Bruxelles. Belgium
Eur. j. anat ; 19(2): 189-195, abr. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-141209
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Caspases are proteases primarily involved in the process of apoptosis; however, caspases can exert non-apoptotic functions. The purpose of this work was to use immunohistochemistry to analyse the expression sites of caspase-2 during normal mouse cephalic development and in embryos exposed to irradiation. Control embryos from embryonic day 9 (E9) to E17 were analysed, and E9 and 10 irradiated embryos were removed and observed after administration of 2 Gy irradiation at embryonic day 9. Surprisingly, not only apoptotic cells expressed caspase-2. In addition, numerous cell populations in normal and experimental embryos displayed transient but intense caspase-2 immunoreactivity, with nuclear and cytoplasmic localisation. This immunoreactivity was not observed with caspase-3 and -9 antibodies. Cranial neural crest cells, premuscular blastemata, cartilage, teeth, the heart, the eye and some other structures displayed caspase-2 expression, with progressive changes during embryonic development. These changing patterns evoke progressive waves of cell differentiation in specific cell populations. Little is known regarding the non-apoptotic functions of caspase-2. Despite the difficulty in understanding the role of this protease during cell differentiation, the fact that caspase-2 is known to prevent DNA damage and to protect the cell cycle could be closely associated with our observations, which point to the need for further research, particularly in caspase-2 knockout mice
RESUMEN
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Assuntos
Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Encéfalo / Imuno-Histoquímica / Caspase 2 Limite: Animais Idioma: Inglês Revista: Eur. j. anat Ano de publicação: 2015 Tipo de documento: Artigo Instituição/País de afiliação: Université Libre de Bruxelles/Belgium
Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Encéfalo / Imuno-Histoquímica / Caspase 2 Limite: Animais Idioma: Inglês Revista: Eur. j. anat Ano de publicação: 2015 Tipo de documento: Artigo Instituição/País de afiliação: Université Libre de Bruxelles/Belgium
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