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Endostatin exerts radiosensitizing effect in non-small cell lung cancer cells by inhibiting VEGFR2 expression
Liu, L; Qiao, Y; Hu, C; Liu, Y; Xia, Y; Wang, L; Liu, B; Chen, H; Jiang, X.
Afiliação
  • Liu, L; Xuzhou Medical College Graduate Academy. Xuzhou. China
  • Qiao, Y; Lianyungang First People’s Hospital. Department of Radiation Oncology. Jiangsu. China
  • Hu, C; Lianyungang First People’s Hospital. Department of Radiation Oncology. Jiangsu. China
  • Liu, Y; Xuzhou Medical College Graduate Academy. Xuzhou. China
  • Xia, Y; Lianyungang First People’s Hospital. Department of Radiation Oncology. China
  • Wang, L; Lianyungang First People’s Hospital. Department of Radiation Oncology. China
  • Liu, B; Xuzhou Medical College Graduate Academy. Xuzhou. China
  • Chen, H; Xuzhou Medical College Graduate Academy. Xuzhou. China
  • Jiang, X; Lianyungang First People’s Hospital. Department of Radiation Oncology. China
Clin. transl. oncol. (Print) ; 18(1): 18-26, ene. 2016. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-148047
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Background. To determine the effects of endostatin on vascular growth factor receptor 2 (VEGFR2) expression in non-small cell lung cancer (NSCLC) cells and the mechanisms underlying its radiosensitizing effect. Methods. VEGFR2 mRNA levels were determined in different NSCLC cell lines using qRT-PCR. RT-PCR and Western blot assays were used to assess the expression of mRNA and proteins. The radiosensitivity of the cells was determined by colony-formation assays; and cell apoptosis and cell cycle distribution were determined by flow cytometry. Results. VEGFR2 mRNA levels differed among the five NSCLC cell lines (P < 0.01), with the highest expression in Calu-1 cells and lowest in A549 cells. Endostatin significantly inhibited the growth of Calu-1 cells (P < 0.01) (IC20 = 296.5 μg/ml), and the expression of VEGFR2 and HIF-1α (P < 0.05). Phosphorylation of protein kinase B (Akt), extracellular signal-regulated kinases 1/2 (ERK1/2), and p38 were significantly lower in endostatin-treated cells than control (P < 0.05). Endostatin enhanced the radiosensitivity of Calu-1 cells to SER = 1.38 and induced apoptosis (P < 0.01) and G2/M blockage (P < 0.01). However, endostatin had limited effects on A549 cells. Compared with Calu-1 cells, there was not significantly effects on cell radiosensitivity (SER = 1.09). Conclusions. Endostatin induces apoptosis and enhances radiosensitivity of the VEGFR2 high-expressing cell line Calu-1, but it has a limited effect on the VEGFR2 low-expressing cell line A549 (AU)
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Coleções: Bases de dados nacionais / Espanha Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Outras Doenças Respiratórias / Neoplasias do Pulmão, Traqueia e Brônquios Base de dados: IBECS Assunto principal: Células Gigantes / Apoptose / Carcinoma Pulmonar de Células não Pequenas / Citometria por Imagem / Endostatinas / Linfonodos Tipo de estudo: Estudo de avaliação Limite: Feminino / Humanos / Masculino País/Região como assunto: Ásia Idioma: Inglês Revista: Clin. transl. oncol. (Print) Ano de publicação: 2016 Tipo de documento: Artigo Instituição/País de afiliação: Lianyungang First People’s Hospital/China / Xuzhou Medical College Graduate Academy/China
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Coleções: Bases de dados nacionais / Espanha Contexto em Saúde: ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis Problema de saúde: Outras Doenças Respiratórias / Neoplasias do Pulmão, Traqueia e Brônquios Base de dados: IBECS Assunto principal: Células Gigantes / Apoptose / Carcinoma Pulmonar de Células não Pequenas / Citometria por Imagem / Endostatinas / Linfonodos Tipo de estudo: Estudo de avaliação Limite: Feminino / Humanos / Masculino País/Região como assunto: Ásia Idioma: Inglês Revista: Clin. transl. oncol. (Print) Ano de publicação: 2016 Tipo de documento: Artigo Instituição/País de afiliação: Lianyungang First People’s Hospital/China / Xuzhou Medical College Graduate Academy/China
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