Specific immune response to Phleum pratense plant profilin in atopic patients and control subjects
Allergol. immunopatol
; 29(1): 9-15, ene. 2001. graf
Artigo
em Inglês
| IBECS
| ID: ibc-150535
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Background:
Phleum pratense (Phl p) pollen is a known cause of allergic disease worldwide. Profilins have been identified as functional plant pan-allergens. The role of Phl p profilin in the specific immune response in sensitized Phl p patients is unknown.Methods:
skin prick test and specific serum IgE levels were performed in 26 patients allergic to Phl p and in 18 nonallergic control donors. Peripheral blood mononuclear cells were isolated from both groups and stimulated with crude extract or highly purified Phl p profilin, and the production of type I and type ll cytokines was determined in patients and controls stimulated with specific and polyclonal stimulus. T-cell lines specific to Phl p profilin were established from PBMCs and cross-reactivity with another highly purified profilin from Parietaria judaica (Pj) was evaluated.Results:
patients allergic to Phl p profilin showed increased T-cell-proliferative responses to this profilin compared with control subjects. The production of IL-4 and IFN-g in response to the specific stimulus was undetectable. However, the production of IL-4 and IFN-g in response to a polyclonal stimulus (PHA) was measurable and different for atopic patients and controlsubjects:
IL-4 was higher (p < 0.001) in allergic patients and IFN-g lower (although not significant) in controls. Neither the T-cell responses nor the production of IL-4 in response to a polyclonal stimulus (PHA) correlated with the individual degree of cutaneous response to Phl p profilin or to the levels of specific Phl p IgE. The T-cell lines tested did not show any cross-reactivity with Pj profilin.Conclusions:
Phl p profilin is in part responsible for the T-cell mediated immunological response in patients allergic to Phl p. The response is very specific since Phl p profilin specific T-cell lines did not show cross-reactivity with a highly homologous profilin from Parietaria judaica (Pj). The lack of correlation between the proliferative T-cell response and polyclonal IL-4 production with allergen-specific serum IgE and SPT probably indicates that some of the responding T-cells may be involved in immune reactions other than the support of IgE (AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.4: Reduzir as mortes prematuras devido doenças não transmissíveis
Base de dados:
IBECS
Assunto principal:
Pólen
/
Proteínas de Vegetais Comestíveis
/
Imunoglobulina E
/
Alérgenos
/
Rinite Alérgica Sazonal
/
Subpopulações de Linfócitos T
/
Hipersensibilidade Imediata
/
Proteínas dos Microfilamentos
Tipo de estudo:
Estudo de etiologia
/
Estudo prognóstico
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2001
Tipo de documento:
Artigo
Instituição/País de afiliação:
Hospital Clínic Barcelona/España
/
Universitat Rovira i Virgili/España
/
University of Basque Country/España