Immunocytochemical evidence for the coexistence of aromatase P450 and estrogen receptor α in the pituitary gland of the adult male rat
Eur. j. anat
; 13(1): 1-6, mayo 2009. ilus
Artigo
em Inglês
| IBECS
| ID: ibc-157848
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
In previous studies we demonstrated the immunocytochemical expression of aromatase in rat pituitary cells. The aim of the present study is to demonstrate the synthesis of the enzyme through the expression of the mRNA of the enzyme in pituitary cells by in situ hybridization and the relationship between the immunocytochemical expression of aromatase and estrogen receptor α in adult male rats. In situ hybridization revealed that aromatase-mRNA is located in the cytoplasm of 41% of pituitary cells. By immunocytochemistry, different patterns of reaction for aromatase were found. Moreover, using immunocytochemistry and RT-PCR, all aromatase-positive tumors proved be estrogen receptor α-positive tumors. The immunocytochemical coexistence of aromatase and estrogen receptor α was found. These observations demonstrate the synthesis of aromatase in the pituitary gland and its functional ability to develop its estrogenic effects mediated by estrogen receptor α (AU)
RESUMEN
No disponible
Buscar no Google
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Imuno-Histoquímica
/
Aromatase
/
Sistema Enzimático do Citocromo P-450
/
Estradiol
/
Animais de Laboratório
Limite:
Animais
Idioma:
Inglês
Revista:
Eur. j. anat
Ano de publicação:
2009
Tipo de documento:
Artigo
Instituição/País de afiliação:
University Pontificia of Salamanca/Spain
/
University of Salamanca/Spain