Could formaldehyde induce mutagenic and cytotoxic effects in buccal epithelial cells during anatomy classes?
Med. oral patol. oral cir. bucal (Internet)
; 22(1): e58-e63, ene. 2017. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-159767
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
BACKGROUND:
Due to increased formaldehyde exposure, carcinogenic to humans, several researches have been studying the potential toxicity and the safe levels for human beings. The aim of this study was to investigate mutagenicity and cytotoxicity in buccal epithelial exfoliated cells (BEC) of students subjected to formaldehyde (FA) during anatomy classes. MATERIAL ANDMETHODS:
BEC were collected periodically from 17 volunteers of undergraduate programs, who had participated in practical anatomy classes, before and after FA exposure. Cells were stained according to Feulgen method and then micronucleus test was applied. A total of 1,500 cells were assessed per individual in this study for the micronucleus frequency and other parameters of cytotoxicity.RESULTS:
There was statistically significant increase in number of micronucleated BEC after FA exposure (after 1 month p=.034 and after 3.5 months p=.017). However, FA exposure caused no significant increase in other nuclear alterations closely related to cytotoxicity (p≥.05).CONCLUSIONS:
FA induced mutagenicity during anatomy classes. Cell death increased, but it was not statistically significant. Efforts have to be made to improve air quality and reduce exposures during anatomy classes
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Citotoxicidade Imunológica
/
Células Epiteliais
/
Formaldeído
Limite:
Humanos
Idioma:
Inglês
Revista:
Med. oral patol. oral cir. bucal (Internet)
Ano de publicação:
2017
Tipo de documento:
Artigo
Instituição/País de afiliação:
Federal University of Espírito Santo/Brazil
/
University of São Paulo/Brazil