Biological function and mechanism of miR-33a in prostate cancer survival and metastasis: via downregulating Engrailed-2
Clin. transl. oncol. (Print)
; 19(5): 562-570, mayo 2017. graf
Artigo
em Inglês
| IBECS
| ID: ibc-162189
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Objective. Recent studies have identified Engrailed-2 (EN-2), a homeobox-containing transcription factor, as a candidate oncogene in prostate cancer (PC). Therapeutic targeting on EN-2, however, is limited because the mechanism underlying EN-2 overexpression in prostatic cancer cells is unknown. This study was to investigate the potential regulatory role of miR-33a on EN-2 expression and explore this signaling axis in ability of prostate cancer survival and metastasis. Methods. The relative expression of miR-33a and EN-2 in paired prostate cancer tissue and adjacent normal tissue as well as in prostate cancer cell lines, PC3 and DU145, was determined using quantitative real-time PCR or western blot, respectively. Cells survival, migration and invasion were evaluated by assays of MTT, TUNEL and Boyden chamber assays, respectively. Direct regulation of EN-2 by miR-33a was examined by luciferase reporter assay. Results. The data showed that miR-33a was upregulated and EN-2 was downregulated in both prostate cancer tissue and prostate cancer cells. miR-33a overexpression suppresses prostate cancer cell survival and metastasis. miR-33a can directly act on EN-2 expression by binding to 3′UTR of its mRNA. Also, miR-33a negatively regulated EN-2 mRNA and protein expression. In pcDNA-EN-2 and miR-33a mimic co-transfected PC3 and DU145 cells, EN-2 overexpression reverses the anti-cell survival and metastasis actions of miR-33a overexpression. The pivotal role of miR-33a in inhibiting prostate tumor growth was confirmed in xenograft models of prostate cancer. Conclusion. Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer. Also in this process EN-2 serves as a negative responder for miR-33a (AU)
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Neoplasias da Próstata
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RNA Mensageiro
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Biomarcadores Tumorais
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Movimento Celular
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Proteína de Suscetibilidade a Apoptose Celular
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Metástase Neoplásica
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
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Masculino
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2017
Tipo de documento:
Artigo
Instituição/País de afiliação:
The First Affiliated Hospital/China
/
Zhengzhou First Peoples Hospital/China
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Zhengzhou University/China