Your browser doesn't support javascript.
loading
Characterization of high exopolysaccharideproducing Lactobacillus strains isolated from mustard pickles for potential probiotic applications
Huang, Jing-Yao; Kao, Cheng-Yen; Liu, We-Sin; Fang, Tony J.
Afiliação
  • Huang, Jing-Yao; National Chung Hsing University. Department of Food Science and Biotechnology. Taichung. República de China
  • Kao, Cheng-Yen; National Yang Ming University. School of Biomedical Science and Engineering. Department of Biotechnology and Laboratory Science in Medicine. Taipei. República de China
  • Liu, We-Sin; National Chung Hsing University. Department of Food Science and Biotechnology. Taichung. República de China
  • Fang, Tony J; Food Industry Research and Development Institute. Hsinchu. República de China
Int. microbiol ; 20(2): 75-84, jun. 2017. graf, tab, ilus
Artigo em Inglês | IBECS | ID: ibc-164432
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
The aim of this study was to characterize high exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) isolated from mustard pickles in República de China for potential probiotic applications. Among 39 collected LAB strains, four most productive EPS-producing strains were selected for further analysis. Comparative analyses of 16S rDNA genes rpoA and pheS sequences demonstrated that these strains were members of Lactobacillus plantarum-group (LPG). NCD 2, NLD 4, SLC 13, and NLD 16 showed survival rates of 95.83% ± 0.49%, 95.07% ± 0.64%, 105.84% ± 0.82%, and 99.65% ± 0.31% under simulated gastrointestinal conditions, respectively. No cytotoxic effects on macrophage RAW 264.7 cells were observed when they were treated with a low dose (1 μg/ml) of stimulants extracted from the tested LAB strains. The production of nitric oxide in RAW 264.7 cells incubated with various LAB stimulants showed a dose-dependent increase. Among the four strains, SLC 13 showed higher inhibitory activity on growth of Enterococcus faecalis (BCRC 12302) and Yersinia enterocolitica (BCRC 10807). NLD 4 showed strong inhibitory activity against Escherichia coli O157H7 (ATCC 43894) as compared with the other three strains. In summary, our results suggest that Lactobacillus pentosus SLC 13 may be a good candidate for probiotic applications and for development of antibacterial compounds (AU)
RESUMEN
No disponible
Assuntos

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Polissacarídeos / Probióticos / Lactobacillus Idioma: Inglês Revista: Int. microbiol Ano de publicação: 2017 Tipo de documento: Artigo Instituição/País de afiliação: Food Industry Research and Development Institute/República de China / National Chung Hsing University/República de China / National Yang Ming University/República de China

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Polissacarídeos / Probióticos / Lactobacillus Idioma: Inglês Revista: Int. microbiol Ano de publicação: 2017 Tipo de documento: Artigo Instituição/País de afiliação: Food Industry Research and Development Institute/República de China / National Chung Hsing University/República de China / National Yang Ming University/República de China
...