Oral immunisation with Taishan Pinus massoniana pollen polysaccharide adjuvant with recombinant Lactococcus lactis-expressing Proteus mirabilis ompA confers optimal protection in mice
Allergol. immunopatol
; 45(5): 496-505, sept.-oct. 2017. graf, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-167005
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Background:
Proteus mirabilis poses a critical burden on the breeding industry, but no efficient vaccine is available for animals.Method:
A recombinant Lactococcus lactis expressing the ompA of P. mirabilis was used to develop a vaccine. The mucosal and systemic immune responses of the recombinant vaccine were evaluated in mice after oral immunisation. The inhibition on P. mirabilis colonisation of vaccines was also determined. Moreover, Taishan Pinus massoniana pollen polysaccharides (TPPPS) were used as adjuvants to examine the immunomodulatory effects.Results:
The pure recombinant L. lactis vaccine significantly induced the production of specific IgA and IgG, IL-2, IL-4, IFN-γ, and T lymphocyte proliferation, and the immunised mice exhibited significant resistance to P. mirabilis colonisation. Notably, the TPPPS adjuvant vaccines induced higher levels of immune responses than the pure L. lactis.Conclusions:
The L. lactis as a vaccine vehicle combined with TPPPS adjuvant provides a feasible method for preventing P. mirabilis infection (AU)RESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
IBECS
Assunto principal:
Infecções por Proteus
/
Proteus mirabilis
/
Vacinas Sintéticas
/
Lactococcus lactis
/
Imunoterapia
Limite:
Animais
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2017
Tipo de documento:
Artigo
Instituição/País de afiliação:
Animal Husbandry and Veterinary Bureau of Shandong Province/China
/
New Hope Group/China
/
Shandong Agricultural University/China