Kaempferol inhibits the production of ROS to modulate OPN-alfavbeta3 integrin pathway in HUVECs
J. physiol. biochem
; 72(2): 303-313, jun. 2016. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-168274
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
In the present study, we tested the hypothesis that aldosterone regulates osteopontin (OPN)-related signaling pathways to promote nuclear factor κB (NF-κB) activation in primary human umbilical vein endothelial cells (HUVECs) and that kaempferol, a flavonoid compound, blocks those changes. Aldosterone induced productions of reactive oxygen species (ROS), OPN, interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) and expression of nicotinamide adenine dinucleotide phosphate-oxidase 4 (Nox4), NF-κB, OPN, alphavbeta3 (αvβ3) integrin, and inhibitor of NF-κB alpha phosphorylation (P-IκBα) in HUVEC. HUVECs were pretreated with kaempferol (0, 1, 3, or 10 μM) for 1 h and exposed to aldosterone (10−6 M) for 24 h. Kaempferol reduced ROS, OPN, NF-κB, IL-6, and TNF-α levels; Nox4, αvβ3 integrin; and P-IκBα expressions. The effect of aldosterone was also abrogated by spironolactone (10−6 M). In addition, vitamin C (20 mmol/L) reduced ROS production. Vitamin C and LM609 (10 μg/mL) treatment decreased expressions of OPN, αvβ3 integrin, and NF-κB (P < 0.05 or P < 0.01). The present results suggest that kaempferol may modulate OPN-αvβ3 integrin pathway to inhibit NF-κB activation in HUVECs (AU)
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Endotélio Vascular
/
Transdução de Sinais
/
Regulação para Baixo
/
Anti-Inflamatórios não Esteroides
/
Espécies Reativas de Oxigênio
/
Quempferóis
/
Antioxidantes
Limite:
Humanos
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Hunan Agricultural University/China
/
Xiangnan University/China