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Effect of total sonicated Aggregatibacter actinomycetemcomitans fragments on gingival stem/progenitor cells
El-Sayed, Karim Fawzy; Graetz, Christian; Köhnlein, Theresa; Mekhemar, Mohamed; Dörfer, Christof.
Afiliação
  • El-Sayed, Karim Fawzy; Cairo University. Faculty of Oral and Dental Medicine. Oral Medicine and Periodontology Department. Egypt
  • Graetz, Christian; Christian Albrechts University. School of Dental Medicine. Clinic for Conservative Dentistry and Periodontology. Kiel. Germany
  • Köhnlein, Theresa; Christian Albrechts University. School of Dental Medicine. Clinic for Conservative Dentistry and Periodontology. Kiel. Germany
  • Mekhemar, Mohamed; Christian Albrechts University. School of Dental Medicine. Clinic for Conservative Dentistry and Periodontology. Kiel. Germany
  • Dörfer, Christof; Christian Albrechts University. School of Dental Medicine. Clinic for Conservative Dentistry and Periodontology. Kiel. Germany
Med. oral patol. oral cir. bucal (Internet) ; 23(5): e569-e578, sept. 2018. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-176376
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT

BACKGROUND:

Aggregatibacter-actinomycetemcomitans (A.actinomycetemcomitans) are strongly associated with localized-aggressive-periodontitis (LAgP). The study's aim was to test for the first time the effect of total sonicated A.actinomycetemcomitans-bacterial-fragments on gingival mesenchymal stem/progenitor cells' (G-MSCs) proliferation and regenerative gene expression in-vitro. MATERIAL AND

METHODS:

G-MSCs were isolated, characterized, expanded and stimulated by total sonicated A.actinomycetemcomitans-bacterial-fragments (0 (negative-control), 15, 60, 120 and 240μg/ml; serovar-b; n=6/group). Cellular proliferation and NF-κβ (NFKB1), Alkaline Phosphatase (ALPL), Collagen-I (COL1A1), Collagen-III (COL3A1), Osteonectin (SPARC) and Osteopontin (SPP1) m-RNA expression were assessed via reverse-transcription-polymerase-chain-reaction (RT-PCR) at 24, 48 and 72 hours and CFUs-ability evaluated at twelve days.

RESULTS:

G-MSCs demonstrated stem/progenitor cells' characteristics. A.actinomycetemcomitans-bacterial-fragments (up to 72 hours) resulted in marked G-MSCs' proliferation over-time (p < 0.001) and elevated NFKB1 (p= 0.017), COL1A1 (p = 0.025), SPARC (p = 0.025), decreased ALPL (p = 0.017), with no significant differences for COL3A1 and SPP1 expression or stimulation times (p > 0.05; Friedman-test). Longer-term stimulation for twelve days reduced G-MSCs' CFUs.

CONCLUSIONS:

Sonicated A.actinomycetemcomitans-bacterial-fragments' exert beneficial short-term effects on G-MSCs' proliferative and non-mineralized tissue forming aptitude. Results shed new light on the importance of periodontal treatment for LAgP patients, using power driven sonic/ultrasonic devices, which, in addition to reducing the subgingival microbial load, produces cell-stimulatory A.actinomycetemcomitans-bacterial-fragments, with positive attributes on tissue reparative/regenerative responses of tissue resident stem/progenitor cells in their niche
Assuntos

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Periodontite / Células-Tronco / Infecções por Pasteurellaceae / Aggregatibacter actinomycetemcomitans / Gengiva Limite: Humanos Idioma: Inglês Revista: Med. oral patol. oral cir. bucal (Internet) Ano de publicação: 2018 Tipo de documento: Artigo Instituição/País de afiliação: Cairo University/Egypt / Christian Albrechts University/Germany

Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Periodontite / Células-Tronco / Infecções por Pasteurellaceae / Aggregatibacter actinomycetemcomitans / Gengiva Limite: Humanos Idioma: Inglês Revista: Med. oral patol. oral cir. bucal (Internet) Ano de publicação: 2018 Tipo de documento: Artigo Instituição/País de afiliação: Cairo University/Egypt / Christian Albrechts University/Germany
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