Your browser doesn't support javascript.
loading
Sodium butyrate protects against oxidative stress in HepG2 cells through modulating Nrf2 pathway and mitochondrial function
Xing, Xingan; Jiang, Zheshu; Tang, Xue; Wang, Panpan; Li, Yingrui; Sun, Yongjuan; Le, Guowei; Zou, Sixiang.
Afiliação
  • Xing, Xingan; Jiangnan University. State Key Laboratoy of Food Science and Tecnology. Wuxi. China
  • Jiang, Zheshu; Wuxi No.1 Senior High School. Wuxi. China
  • Tang, Xue; Jiangnan University. State Key Laboratoy of Food Science and Tecnology. Wuxi. China
  • Wang, Panpan; Jiangnan University. School of Food Science and Technology. Wuxi. China
  • Li, Yingrui; Jiangnan University. School of Food Science and Technology. Wuxi. China
  • Sun, Yongjuan; Jiangnan University. School of Food Science and Technology. Wuxi. China
  • Le, Guowei; Jiangnan University. State Key Laboratoy of Food Science and Tecnology. Wuxi. China
  • Zou, Sixiang; Nanjing Agricultural University. Key Laboratory of Animal Physiology and Biochemistry. Nanjing. China
J. physiol. biochem ; 73(3): 405-414, ago. 2017. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-178892
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Sodium butyrate (NaBu) is a by-product of microbial fermentation of dietary fiber in the gastrointestinal tract and has been shown to increase the activity of antioxidant enzymes, such as catalase or heme oxidase-1, in vivo. However, the mechanism of this effect is still unclear. This study investigated the antioxidant effect of NaBu on HepG2 cells under H2O2-induced oxidative stress. NaBu (0.3 mM) attenuated cell death and accumulation of reactive oxygen species and improved multiple antioxidant parameters in H2O2-injured HepG2 cells. NaBu inhibited glycogen synthase kinase-3 beta (GSK-3Beta) by increasing the p-GSK-3 Beta (Ser9) level and promoted nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), which increased the expression of downstream antioxidant enzymes. Together with promotion of peroxisome proliferator-activated receptor gamma coactivator 1-alpha and mitochondrial DNA copy number, NaBu modulated energy metabolism and mitochondrial function, decreasing glycolysis, increasing Beta -oxidation, and enhancing the tricarboxylic acid cycle and oxidative phosphorylation. NaBu increased mitochondrial manganese-superoxide dismutase and glutathione peroxidase activity. In conclusion, NaBu protected HepG2 cells against oxidative stress by modulating Nrf2 pathway activity and mitochondrial function
Assuntos
Buscar no Google
Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Sobrevivência Celular / Estresse Oxidativo / Ácido Butírico / Fator 2 Relacionado a NF-E2 / Variações do Número de Cópias de DNA / Mitocôndrias Limite: Humanos Idioma: Espanhol Revista: J. physiol. biochem Ano de publicação: 2017 Tipo de documento: Artigo Instituição/País de afiliação: Jiangnan University/China / Nanjing Agricultural University/China / Wuxi No.1 Senior High School/China
Buscar no Google
Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Sobrevivência Celular / Estresse Oxidativo / Ácido Butírico / Fator 2 Relacionado a NF-E2 / Variações do Número de Cópias de DNA / Mitocôndrias Limite: Humanos Idioma: Espanhol Revista: J. physiol. biochem Ano de publicação: 2017 Tipo de documento: Artigo Instituição/País de afiliação: Jiangnan University/China / Nanjing Agricultural University/China / Wuxi No.1 Senior High School/China
...