Sodium butyrate protects against oxidative stress in HepG2 cells through modulating Nrf2 pathway and mitochondrial function
J. physiol. biochem
; 73(3): 405-414, ago. 2017. graf, tab
Artigo
em Espanhol
| IBECS
| ID: ibc-178892
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Sodium butyrate (NaBu) is a by-product of microbial fermentation of dietary fiber in the gastrointestinal tract and has been shown to increase the activity of antioxidant enzymes, such as catalase or heme oxidase-1, in vivo. However, the mechanism of this effect is still unclear. This study investigated the antioxidant effect of NaBu on HepG2 cells under H2O2-induced oxidative stress. NaBu (0.3 mM) attenuated cell death and accumulation of reactive oxygen species and improved multiple antioxidant parameters in H2O2-injured HepG2 cells. NaBu inhibited glycogen synthase kinase-3 beta (GSK-3Beta) by increasing the p-GSK-3 Beta (Ser9) level and promoted nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2), which increased the expression of downstream antioxidant enzymes. Together with promotion of peroxisome proliferator-activated receptor gamma coactivator 1-alpha and mitochondrial DNA copy number, NaBu modulated energy metabolism and mitochondrial function, decreasing glycolysis, increasing Beta -oxidation, and enhancing the tricarboxylic acid cycle and oxidative phosphorylation. NaBu increased mitochondrial manganese-superoxide dismutase and glutathione peroxidase activity. In conclusion, NaBu protected HepG2 cells against oxidative stress by modulating Nrf2 pathway activity and mitochondrial function
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Sobrevivência Celular
/
Estresse Oxidativo
/
Ácido Butírico
/
Fator 2 Relacionado a NF-E2
/
Variações do Número de Cópias de DNA
/
Mitocôndrias
Limite:
Humanos
Idioma:
Espanhol
Revista:
J. physiol. biochem
Ano de publicação:
2017
Tipo de documento:
Artigo
Instituição/País de afiliação:
Jiangnan University/China
/
Nanjing Agricultural University/China
/
Wuxi No.1 Senior High School/China