Single-tube classical PCR for Candida auris and Candida haemulonii identification / PCR múltiple para la identificación de Candida auris y Candida haemulonii
Rev. iberoam. micol
; 35(2): 110-112, abr.-jun. 2018. ilus
Artigo
em Inglês
| IBECS
| ID: ibc-179568
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Background:
Candida auris and Candida haemulonii are emerging and multiresistant pathogens. C. auris has produced hospital outbreaks and is misidentified by phenotypic-based methods. The only reliable identification methods are DNA sequencing and MALDI-TOF.Aims:
To develop a classical-PCR method capable of rapidly and accurately identify C. auris and C. haemulonii.Methods:
A multiplex PCR was carried out in one tube that included an internal control and oligonucleotides that specifically hybridize to the ITS2 region of C. auris and C. haemulonii. The usefulness of the new method was verified by testing a collection of 50 strains of 20 different species (previously identified by ITS sequencing). The selection of species was made in order to emulate the C. auris panel used by the CDC to validate diagnostic tools. In addition, other yeast species not included in the aforementioned panel were incorporated based on reported identification errors.Results:
The results obtained with the proposed protocol were in total agreement with those obtained by ITS sequencing.Conclusions:
We present a PCR method able to unequivocally identify C. auris and differentiate it from C. haemulonii. It is inexpensive, fast and it could be a useful tool to reduce the chances of a C. auris outbreak
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Candida
/
Candidíase
/
Técnicas de Tipagem Micológica
/
Reação em Cadeia da Polimerase Multiplex
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. iberoam. micol
Ano de publicação:
2018
Tipo de documento:
Artigo
Instituição/País de afiliação:
Centro de Investigaciones Microbiológicas del Cesar (CIMCE) Ltda/Colombia
/
Clínica General del Norte/Colombia
/
Corporación para Investigaciones Biológicas (CIB)/Colombia
/
Universidad Nacional del Litoral/Argentina
/
Universidad Popular del Cesar/Colombia