The effect of neonatal maternal separation on short-chain fatty acids and airway inflammation in adult asthma mice
Allergol. immunopatol
; 47(1): 2-11, ene.-feb. 2019. graf, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-180764
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Background:
To investigate neonatal maternal separation (NMS) effects on airway inflammation of asthma and potential mechanism using a mouse model.Methods:
80 Balb/c neonatal male mice were randomly assigned to NMS and non-NMS groups. Feces were collected on PND21, 28, 35 and 42 to analyze microbiota and short-chain fatty acids (SCFAs). Non-NMS group were then divided into control (group A) and asthma groups (group B), while NMS group was assigned to NMS + asthma (group C) and NMS + SCFAs + asthma groups (group D). Inflammatory cells and eosinophils (EOS) in bronchoalveolar lavage fluid (BALF) were assessed. Pathological changes and cytokines in lung tissue were observed. Protein expression of Occludin and E-cadherin in airway epithelial was examined.Results:
The number of S', diversity index H′ and dominance index D', as well as content butyric acid in NMS group C were significantly lower than non-NMS group B (p<0.05). Mice in group C had a higher level of inflammatory cells and EOS compared with group A, B and D. EOS moderate infiltration was found in mice of group B, C and D. Mice in group C had significantly higher levels of cytokines and showed slightly increased bronchial epithelium goblet cells and a small amount of visceral secretions. Occludin and E-cadherin expression in lung in B, C and D groups was depressed, and protein level in group C was significantly lower than group B and D.Conclusions:
NMS is associated with exacerbated inflammation of adult asthma by changing intestinal microflora resulting in butanoic acid decline and airway epithelial barrier damageRESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Sistema Respiratório
/
Asma
/
Eosinófilos
/
Fezes
/
Inflamação
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2019
Tipo de documento:
Artigo
Instituição/País de afiliação:
Southeast University/China
/
Suzhou Municipal Hospital/China