Vitamin B3 impairs reverse cholesterol transport in Apolipoprotein E-deficient mice / La vitamina B3 altera el transporte reverso del colesterol en ratones con deficiencia en apolipoproteína E
Clín. investig. arterioscler. (Ed. impr.)
; 31(6): 251-260, nov.-dic. 2019. tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-185150
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Introduction:
High Density Lipoproteins (HDL) are dysfunctional in hypercholesterolemia patients. The hypothesis was tested that nicotinamide (NAM) administration will influence HDL metabolism and reverse cholesterol transport from macrophages to the liver and feces in vivo (m-RCT) in a murine model of hypercholesterolemia.Methods:
Apolipoprotein E-deficient (KOE) mice were challenged with a high-fat diet for 4 weeks. The effect of different doses of NAM on cholesterol metabolism, and the ability of HDL to promote m-RCT was assessed.Results:
The administration of NAM to KOE mice produced an increase (∼1.5-fold; P < 0.05) in the plasma levels of cholesterol, which was mainly accounted for by the non-HDL fraction. NAM produced a [3H]-cholesterol plasma accumulation (∼1.5-fold) in the m-RCT setting. As revealed by kinetic analysis, the latter was mainly explained by an impaired clearance of circulating non-HDL (∼0.8-fold). The relative content of [3H]-tracer was lowered in the livers (∼0.6-fold) and feces (> 0.5-fold) of NAM-treated mice. This finding was accompanied by a significant (or trend close to significance) up-regulation of the relative gene expression of Abcg5 and Abcg8 in the liver (Abcg5 2.9-fold; P < 0.05; Abcg8 2.4-fold; P = 0.06) and small intestine (Abcg5 2.1-fold; P = 0.15; Abcg8 1.9-fold; P < 0.05) of high-dose, NAM-treated mice.Conclusion:
The data from this study show that the administration of NAM to KOE mice impaired m-RCT in vivo. This finding was partly due to a defective hepatic clearance of plasma non-HDLRESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Apolipoproteínas E
/
Colesterol
/
Niacinamida
/
Hipercolesterolemia
Limite:
Animais
Idioma:
Inglês
Revista:
Clín. investig. arterioscler. (Ed. impr.)
Ano de publicação:
2019
Tipo de documento:
Artigo
Instituição/País de afiliação:
CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)/Spain
/
CIBER de Diabetes y Enfermedades Metabólicas Asociadas/Spain
/
Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau i Institut dInvestigació Biomèdica de lHospital de la Santa Creu i Sant Pau/Spain
/
Institut de Recerca de lHospital de la Santa Creu i Sant Pau i Institut dInvestigació Biomèdica de l'Hospital de la Santa Creu i Sant Pau/Spain
/
Universitat Autònoma de Barcelona/Spain