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Reduced Akkermansia muciniphila and Faecalibacterium prausnitzii levels in the gut microbiota of children with allergic asthma
Demirci, M; Tokman, HB; Uysal, HK; Demiryas, S; Karakullukcu, A; Saribas, S; Cokugras, H; Kocazeybek, BS.
Afiliação
  • Demirci, M; Beykent University. Medical Faculty. Department of Medical Microbiology. Istanbul. Turkey
  • Tokman, HB; Istanbul University-Cerrahpaşa. Cerrahpaşa Medical Faculty. Department of Medical Microbiology. Istanbul. Turkey
  • Uysal, HK; Istanbul University. Istanbul Medical Faculty. Department of Medical Microbiology. Istanbul. Turkey
  • Demiryas, S; Istanbul University-Cerrahpaşa. Cerrahpaşa Medical Faculty. Department of General Surgery. Istanbul. Turkey
  • Karakullukcu, A; Gümüşhane University. Vocational School of Health Services. Gümüşhane. Turkey
  • Saribas, S; Istanbul University-Cerrahpaşa. Cerrahpaşa Medical Faculty. Department of Medical Microbiology. Istanbul. Turkey
  • Cokugras, H; Istanbul University-Cerrahpaşa. Cerrahpaşa Medical Faculty. Department of Pediatric Infectious Diseases, Clinical Immunology and Allergy. Istanbul. Turkey
  • Kocazeybek, BS; Istanbul University-Cerrahpaşa. Cerrahpaşa Medical Faculty. Department of Medical Microbiology. Istanbul. Turkey
Allergol. immunopatol ; 47(4): 365-371, jul.-ago. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-186508
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Introduction and

objectives:

The amounts of Akkermansia muciniphila and Faecalibacterium prausnitzii in gut microbiota are reduced in patients with allergic diseases compared to healthy controls. We aimed to quantify levels of A. muciniphila and F. prausnitzii amounts using real-time quantitative PCR (qPCR) in the gut microbiota of children with allergic asthma and in healthy controls. Materials and

methods:

In total, 92 children between the ages of three and eight who were diagnosed with asthma and 88 healthy children were included in the study and bacterial DNA was isolated from the stool samples using the stool DNA isolation Kit. qPCR assays were studied with the microbial DNA qPCR Kit for A. muciniphila and microbial DNA qPCR Kit for F. prausnitzii.

Results:

Both bacterial species showed a reduction in the patient group compared to healthy controls. A. muciniphila and F. prausnitzii were found to be 5.45 ± 0.004, 6.74 ± 0.01 and 5.71 ± 0.002, 7.28 ± 0.009 in the stool samples of the asthma and healthy control groups, respectively.

Conclusions:

F. prausnitzii and A. muciniphila may have induced anti-inflammatory cytokine IL-10 and prevented the secretion of pro-inflammatory cytokines like IL-12. These findings suggest that A. muciniphila and F. prausnitzii may suppress inflammation through its secreted metabolites
RESUMEN
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Assuntos
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Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Asma / DNA Bacteriano / Eosinófilos / Fezes / Verrucomicrobia / Microbioma Gastrointestinal / Faecalibacterium prausnitzii Limite: Criança / Criança, pré-escolar / Feminino / Humanos / Masculino Idioma: Inglês Revista: Allergol. immunopatol Ano de publicação: 2019 Tipo de documento: Artigo Instituição/País de afiliação: Beykent University/Turkey / Gümüşhane University/Turkey / Istanbul University/Turkey / Istanbul University-Cerrahpaşa/Turkey
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Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Asma / DNA Bacteriano / Eosinófilos / Fezes / Verrucomicrobia / Microbioma Gastrointestinal / Faecalibacterium prausnitzii Limite: Criança / Criança, pré-escolar / Feminino / Humanos / Masculino Idioma: Inglês Revista: Allergol. immunopatol Ano de publicação: 2019 Tipo de documento: Artigo Instituição/País de afiliação: Beykent University/Turkey / Gümüşhane University/Turkey / Istanbul University/Turkey / Istanbul University-Cerrahpaşa/Turkey
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