Effect of ginger extract on expression of GATA3, T-bet and ROR-yt in peripheral blood mononuclear cells of patients with Allergic Asthma
Allergol. immunopatol
; 47(4): 378-385, jul.-ago. 2019. tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-186510
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Introduction and objectives:
Allergic asthma is a chronic inflammatory disorder of the airways. Th1, Th2 and Th17 cells are the main cells involved in the pathophysiology of asthma. The function of these cells is affected by T-bet, GATA3 and RORgammat transcription factors (respectively). Therefore, the aim of this study was to evaluate the effect of ginger (officinal Roscoe) extract on the expression of T-bet, GATA-3 and ROR-gamma in peripheral blood mononuclear cells (PBMC) of asthmatic patients, in comparison with healthy volunteers as controls. Materials andmethods:
In this case-control study, a total of 50 individuals including 25 patients with severe, moderate and mild allergic asthma and 25 unrelated healthy controls were involved. The PBMCs were isolated and divided into four groups negative control, two positive controls (Budesonide and PHA) and ginger-extract treated group. After cell treatment and incubation for 48h, PBMCs were isolated and cDNA was synthesized. Gene expressions of T-bet, GATA3 and ROR-γt were evaluated by Real-time PCR.Results:
According to the results of this study, hydroalcoholic extract of ginger could reduce the expression of GATA-3, ROR-gammat, and T-bet in PBMCs of asthmatic patients in comparison with untreated PBMCs (P values = 0.001, 0.001, and 0.002, respectively). It was also shown that the ginger extract could affect T-bet/GATA-3, T-bet/ROR-gamma, and ROR-gammat/GATA-3 expression ratios.Conclusions:
This study showed that the use of ginger extract could control asthma and decrease the severity of this disease by affecting the main cells involving the symptoms of asthma in the airwaysRESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Asma
/
Leucócitos Mononucleares
/
Extratos Vegetais
/
Antiasmáticos
/
Proteínas com Domínio T
/
Fator de Transcrição GATA3
/
Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares
/
Hipersensibilidade
Limite:
Adolescente
/
Adulto
/
Criança
/
Humanos
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2019
Tipo de documento:
Artigo
Instituição/País de afiliação:
Mazandaran University of Medical Sciences/Iran